Department of Clinical Medicine, Jining Medical Universitygrid.449428.7, Jining, Shandong, China.
Laboratory of Medical Mycology, Jining No. 1 People's Hospital, Jining, Shandong, China.
Microbiol Spectr. 2022 Feb 23;10(1):e0116921. doi: 10.1128/spectrum.01169-21. Epub 2022 Jan 12.
Seborrheic dermatitis (SD) is a common, chronic, and relapsing skin disease. The roles of spp. in the pathogenesis of SD are still not clear due to the lack of direct evidence for the existence of hyphae within affected skin tissues. We set out to elucidate if mycelium contributes to the onset and development of SD and if mycelium is correlated with the clinical severity of SD patients. We detected hyphae in patients with SD using potassium hydroxide (KOH) and calcofluor white (CFW) staining. Fluorescent microscopy was performed for the analysis of fungal cell wall and morphological characteristics of under CFW staining. Culture growth in modified Dixon agar was used for DNA extraction and sequencing, and species were confirmed by a sequencing data BLAST search against the NCBI database. We demonstrated that hyphae were positively correlated with the clinical severity of SD patients (= 3.1738 × 10). All the patients responded well to antifungal treatment. There is no significant difference for species dominance across the variant groups. However, the exact molecular mechanisms of how spp. affect SD need to be further explored. The results show that spp. in the hyphal stage are restricted to SD patients compared with healthy controls, suggesting that the presence of hyphae contributes to the pathogenesis of SD. The results highlight the importance of the antifungal therapy for the future treatment of SD patients. Our results support the proposal that the hyphal form of could be one of the pathogenic factors that contribute to SD, which has been previously less well studied. This clinical observation paves the way for further investigations of the molecular mechanisms of hyphal pathogenicity in SD.
脂溢性皮炎（SD）是一种常见的慢性复发性皮肤病。由于缺乏真菌在受影响皮肤组织内存在的菌丝的直接证据，因此 spp. 在 SD 发病机制中的作用仍不清楚。我们着手阐明菌丝是否有助于 SD 的发生和发展，以及菌丝是否与 SD 患者的临床严重程度相关。我们使用氢氧化钾（KOH）和钙荧光白（CFW）染色检测 SD 患者的菌丝。荧光显微镜用于分析 CFW 染色下真菌细胞壁和形态特征。改良 Dixon 琼脂培养用于 DNA 提取和测序，并通过与 NCBI 数据库的测序数据 BLAST 搜索来确认 种。我们表明，菌丝与 SD 患者的临床严重程度呈正相关（= 3.1738 × 10）。所有患者对抗真菌治疗反应良好。在不同变异组中，种的优势没有显著差异。然而， spp. 如何影响 SD 的确切分子机制仍需进一步探索。结果表明，与健康对照组相比，SD 患者中处于菌丝期的 spp. 受到限制，这表明菌丝的存在有助于 SD 的发病机制。这些结果强调了未来治疗 SD 患者时进行抗真菌治疗的重要性。我们的研究结果支持这样的观点，即 的菌丝形式可能是导致 SD 的致病因素之一，这在以前的研究中较少涉及。这一临床观察为进一步研究 SD 中 菌丝致病性的分子机制铺平了道路。
