Ng C S, Chan J K, Lo S T, Lo D S
Department of Morbid Anatomy, Prince of Wales Hospital.
Histopathology. 1987 Dec;11(12):1243-58. doi: 10.1111/j.1365-2559.1987.tb01870.x.
Four commercially available monoclonal antibodies, MB1, MB2, LN1 and LN2, were studied to determine their sensitivity and specificity for the diagnosis of B-cell lymphomas when used on formalin-fixed paraffin-embedded tissues. In addition to 125 cases of immunologically characterized non-Hodgkin's lymphoma, a range of normal tissues, reactive lymphoid proliferations, Hodgkin's disease and granulocytic sarcomas were also studied. MB1 was found to give positive results in 53.6% of B-cell lymphomas, but the staining was sometimes weak and patchy; there was also cross-reaction with 1.8% of T-cell lymphomas. MB2 reacted with 88.4% of B-cell lymphomas and the reaction was often strong and diffuse, but it showed cross-reaction with 18.2% of T-cell lymphomas. LN1 and LN2 gave positive staining of 44.9 and 46.4% of B-cell lymphomas respectively, and the results appeared to be inferior to that obtained in B5-fixed tissues; staining was sometimes weak and focal, and they also gave false-positive results in a few cases of T-cell lymphoma. This study shows that MB1, LN1 and LN2 are fairly but not entirely specific for B-cells in the non-Hodgkin's lymphomas, but are not very sensitive when applied to formalin-fixed tissues. MB2 shows a high sensitivity but only moderate specificity. Therefore, when these antibodies are used to determine the immunophenotype of malignant lymphomas, the B-cell nature can be predicted with great confidence only when two, preferably three or more, of the antibodies give positive results. The potential applications of these antibodies are discussed.
研究了四种市售单克隆抗体MB1、MB2、LN1和LN2，以确定它们在福尔马林固定石蜡包埋组织上用于诊断B细胞淋巴瘤时的敏感性和特异性。除了125例经免疫特征化的非霍奇金淋巴瘤外，还研究了一系列正常组织、反应性淋巴组织增生、霍奇金病和粒细胞肉瘤。发现MB1在53.6%的B细胞淋巴瘤中呈阳性结果，但染色有时较弱且呈斑片状；也与1.8%的T细胞淋巴瘤有交叉反应。MB2与88.4%的B细胞淋巴瘤发生反应，反应通常强烈且弥漫，但与18.2%的T细胞淋巴瘤有交叉反应。LN1和LN2分别在44.9%和46.4%的B细胞淋巴瘤中呈阳性染色，结果似乎不如在B5固定组织中获得的结果；染色有时较弱且呈局灶性，在少数T细胞淋巴瘤病例中也出现假阳性结果。这项研究表明，MB1、LN1和LN2在非霍奇金淋巴瘤中对B细胞有一定但不完全的特异性，但应用于福尔马林固定组织时敏感性不高。MB2显示出高敏感性但只有中等特异性。因此，当使用这些抗体来确定恶性淋巴瘤的免疫表型时，只有当两种，最好是三种或更多抗体呈阳性结果时，才能非常有把握地预测B细胞性质。讨论了这些抗体的潜在应用。
