Zakhireh Solmaz, Omidi Yadollah, Beygi-Khosrowshahi Younes, Barzegari Abolfazl, Barar Jaleh, Adibkia Khosro
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, Fort Lauderdale, FL 33328, USA.
J Trace Elem Med Biol. 2022 May;71:126921. doi: 10.1016/j.jtemb.2022.126921. Epub 2022 Jan 5.
Cell-adhesive surfaces play a pivotal role in biomedical engineering, as most biological reactions take place on surfaces. Pollen shell (PSh) ofPistacia vera L., as a new medical device, has previously been reported to cause cytotoxicity and apoptosis in MG-63 bone cancer cells.
Iron oxide nanoparticles (FeONPs) were synthesized and their reaction to PShs was gauged at different concentrations, and then characterized using field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy, energy dispersion X-ray spectrometer, X-ray diffraction spectra, dynamic light scattering, and vibrating sample magnetometer. Then, the biological impacts of PShs/FeONPs composites on MG-63 cells were investigated in-vitro using MTT assay, quantitative polymerase chain reaction (qPCR), Annexin V/propidium iodide, FESEM, and DAPI staining.
FeONPs with a size range of 24-40 nm and a zeta potential value of -37.4 mV were successfully assembled on the PShs. The viability of MG-63 cells was significantly decreased when cultured on the magnetic PShs as compared to non-magnetic PShs, in FeO concentration and time-dependent manner. In contrast, magnetic PShs had a positive effect on the viability of normal human bone marrow-derived mesenchymal stem cells (hBM-MSCs). The analysis of apoptosis-related genes in cancer cells revealed that loading FeONPs on PShs increased expression of BAX/BCL2 and caspase-3 genes. The increased apoptotic activity of combined PShs/FeONPs was further confirmed by flow cytometric measurement, morphological analysis, and DAPI staining.
The incorporation of FeONPs into PShs could effectively increase anticancer effects on MG-63 cells via the mitochondria-mediated apoptosis pathway, evident by upregulation of BAX/BCL2 ratio and caspase-3.
细胞粘附表面在生物医学工程中起着关键作用，因为大多数生物反应都发生在表面。阿月浑子的花粉壳（PSh）作为一种新型医疗器械，此前已有报道称其可导致MG-63骨癌细胞发生细胞毒性和凋亡。
合成了氧化铁纳米颗粒（FeONPs），并在不同浓度下测定其与PShs的反应，然后用场发射扫描电子显微镜（FESEM）、傅里叶变换红外光谱、能量色散X射线光谱仪、X射线衍射光谱、动态光散射和振动样品磁强计对其进行表征。然后，使用MTT法、定量聚合酶链反应（qPCR）、膜联蛋白V/碘化丙啶、FESEM和DAPI染色在体外研究PShs/FeONPs复合材料对MG-63细胞的生物学影响。
成功地在PShs上组装了尺寸范围为24-40 nm、ζ电位值为-37.4 mV的FeONPs。与非磁性PShs相比，在磁性PShs上培养时，MG-63细胞的活力以FeO浓度和时间依赖性方式显著降低。相反，磁性PShs对正常人骨髓来源的间充质干细胞（hBM-MSCs）的活力有积极影响。对癌细胞中凋亡相关基因的分析表明，在PShs上负载FeONPs可增加BAX/BCL2和caspase-3基因的表达。流式细胞术测量、形态学分析和DAPI染色进一步证实了PShs/FeONPs组合的凋亡活性增加。
将FeONPs掺入PShs可通过线粒体介导的凋亡途径有效增强对MG-63细胞的抗癌作用，BAX/BCL2比值和caspase-3的上调证明了这一点。
