基于免疫荧光的方法评估肿瘤缺氧区域的癌症标志物。
Immunofluorescence-Based Method to Assess Cancer Biomarker in the Hypoxic Region of the Tumor.
机构信息
Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC, USA.
Julius L. Chambers Biomedical Biotechnology Research Institute, North Carolina Central University, Durham, NC, USA.
出版信息
Methods Mol Biol. 2022;2413:37-43. doi: 10.1007/978-1-0716-1896-7_5.
The development of novel imaging technologies allows the analysis of the expression and spatial distribution of multiple markers simultaneously, providing necessary information about a cellular identity and the surrounding microenvironment. This chapter describes the utilization of immunofluorescence to identify such biomarkers in fixed tissue from prostate cancer (PCa) xenografts. One such marker detectable by immunofluorescence is pimonidazole, which has been utilized to locate areas of low oxygen (hypoxia). Pimonidazole, in combination with other biomarkers, could be utilized to identify "niches" in the microenvironment harboring more aggressive cells both within and outside hypoxic areas. Specifically, we describe the method to use pimonidazole for the identification of hypoxic regions in PCa xenograft tumors along with CPT1A (carnitine palmitoyltransferase 1A) expression, an indicator of β-oxidation. This approach could be useful to characterize various biomarkers in the complex hypoxic tumor microenvironment.
新型成像技术的发展使得同时分析多个标志物的表达和空间分布成为可能，为细胞身份和周围微环境提供了必要的信息。本章描述了利用免疫荧光技术来鉴定前列腺癌（PCa）异种移植固定组织中的此类生物标志物。可通过免疫荧光检测到的一种标志物是 pimonidazole，它已被用于定位低氧（缺氧）区域。pimonidazole 与其他生物标志物结合使用，可用于鉴定缺氧区域内外的微环境“龛位”，其中包含侵袭性更强的细胞。具体来说，我们描述了使用 pimonidazole 来鉴定 PCa 异种移植肿瘤中缺氧区域的方法，以及 CPT1A（肉毒碱棕榈酰转移酶 1A）的表达，CPT1A 是β-氧化的一个指标。这种方法可用于对复杂的缺氧肿瘤微环境中的各种生物标志物进行特征描述。
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