洗必泰与有机硒对抑制变异链球菌生物膜的比较：一项体外研究。

Chlorhexidine versus organoselenium for inhibition of S. mutans biofilm, an in vitro study.

机构信息

Department of Orthodontics, Faculty of Dentistry, Alexandria University, Champollion St., Azarita, P. O. Box: 21521, Alexandria, Egypt.

Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt.

出版信息

BMC Oral Health. 2022 Jan 20;22(1):14. doi: 10.1186/s12903-022-02049-w.

DOI:10.1186/s12903-022-02049-w

PMID:35057785

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8780763/

Abstract

BACKGROUND

Chemical Plaque control by antimicrobial agent application can defend the teeth against caries. S. mutans is considered the main etiologic factor for caries. This was an in vitro study to compare between the efficacy of chlorhexidine diaceteate varnish, and an organoselenium sealant, to prevent S. mutans biofilm formation on human teeth.

METHODS

Fourty five premolars extracted for orthodontic purposes were randomly divided into 3 groups of 15 teeth each. One control group and two test groups, chlorhexidine diaceteate varnish and an organoselenium sealant. The teeth were autoclaved before S. mutans biofilm was induced on to each in their respective groups. The reading T1 was taken for each tooth to assess the number of S. mutans attached in order to compare for differences in surface area among the 3 groups. The respective test materials were applied onto the teeth and biofilm induced onto them in their respective groups. The reading T2 was taken for the 2 test groups. The 3 groups were then subjected to aging for a period equivalent to 5 months before the biofilm was induced to take the reading T3 for the number of S. mutans. We used vortexing of the teeth to disrupt the biofilm at time points T1, T2 and T3. S. mutans count was then done using PCR.

RESULTS

There were significantly lower S. mutans counts in the control group as compared to the chlorhexidine diacetate group at T3.There were no other statistically significant differences found.

CONCLUSION

Both organoselenium and Chlorhexidine diacetate do not inhibit S. mutans biofilm attachment onto the teeth.

摘要

背景

通过使用抗菌剂控制化学菌斑，可以保护牙齿免受龋齿侵害。变形链球菌被认为是龋齿的主要病因。本研究旨在比较乙酸氯己定涂料和有机硒密封剂在预防变形链球菌生物膜形成于人类牙齿表面的效果。

方法

45 颗因正畸需要而拔除的前磨牙被随机分为 3 组，每组 15 颗。一组为对照组，两组为实验组，即乙酸氯己定涂料组和有机硒密封剂组。在诱导变形链球菌生物膜于各组牙齿表面形成之前，先对牙齿进行高压灭菌。记录每个牙齿上的 T1 读数，以评估附着的变形链球菌数量，从而比较三组牙齿表面面积的差异。将相应的测试材料应用于各组牙齿表面，诱导生物膜形成。对两个实验组进行 T2 读数。然后，三组牙齿进行老化处理，相当于 5 个月，然后再次诱导生物膜，以记录 T3 时的变形链球菌数量。在 T1、T2 和 T3 时，我们通过牙齿涡旋来破坏生物膜。然后使用 PCR 检测变形链球菌的数量。