International Joint Research Laboratory for Biointerface and Biodetection, State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
International Joint Research Center for Photo-responsive Molecules and Materials, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
J Am Chem Soc. 2022 Feb 2;144(4):1580-1588. doi: 10.1021/jacs.1c09986. Epub 2022 Jan 21.
Biological application of chiral nanoparticles (NPs) has aroused enormous levels of attention over recent years. Here, we synthesized magneto-chiral cobalt hydroxide (Co(OH)) NPs that exhibited strong chiroptical and unique magnetic properties and applied these NPs to detect and monitor reactive oxygen species (ROS) in living cells and . Circular dichroism (CD) and magnetic resonance imaging (MRI) signals of the magneto-chiral Co(OH) NPs exhibited a wide intracellular ROS detection range from 0.673 to 612.971 pmol/10 cells with corresponding limits of detection (LOD) at 0.087 and 0.179 pmol/10 cells, far below that of currently available probes; the LOD for d-aspartic acid coated Co(OH) NPs (d-Co(OH) NPs) was 5.7 times lower than that for l-aspartic acid coated Co(OH) NPs (l-Co(OH) NPs) based on the CD signals. In addition, d-Co(OH) NPs also exhibited dynamic ROS monitoring ability. The high levels of selectivity and sensitivity to ROS in complex biological environments can be attributed to the Co oxidation reaction on the surface of the NPs. Furthermore, magneto-chiral Co(OH) NPs were able to quantify the levels of ROS in living mice by fluorescence and MRI signals. Collectively, these results reveal that magneto-chiral Co(OH) NPs exhibit a remarkable ability to quantify ROS levels in living organisms, and could therefore provide new tools for exploring chiral nanomaterials as a potential biosensor to investigate biological events.
手性纳米粒子（NPs）在生物领域的应用近年来引起了广泛关注。在此，我们合成了具有强手性光学和独特磁学性质的磁手性钴氢氧化物（Co(OH)） NPs，并将这些 NPs 应用于检测和监测活细胞内的活性氧物种（ROS）。磁手性 Co(OH) NPs 的圆二色性（CD）和磁共振成像（MRI）信号显示出广泛的细胞内 ROS 检测范围，从 0.673 到 612.971 pmol/10 个细胞，相应的检测限（LOD）分别为 0.087 和 0.179 pmol/10 个细胞，远低于目前可用的探针；基于 CD 信号，d-天冬氨酸包裹的 Co(OH) NPs（d-Co(OH) NPs）的 LOD 比 l-天冬氨酸包裹的 Co(OH) NPs（l-Co(OH) NPs）低 5.7 倍。此外，d-Co(OH) NPs 还表现出动态 ROS 监测能力。在复杂的生物环境中，对 ROS 具有高选择性和灵敏度归因于 NPs 表面的 Co 氧化反应。此外，磁手性 Co(OH) NPs 能够通过荧光和 MRI 信号定量活体小鼠中的 ROS 水平。综上所述，这些结果表明磁手性 Co(OH) NPs 具有定量活体生物中 ROS 水平的显著能力，因此可以为探索手性纳米材料作为潜在生物传感器来研究生物事件提供新工具。
