Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
Department of Industrial Engineering, BIOtech Research Center, University of Trento, Via delle Regole 101, 38123, Trento, Italy.
Mikrochim Acta. 2022 Jan 21;189(2):66. doi: 10.1007/s00604-022-05165-0.
The possibility to prepare molecularly imprinted nanoparticles from silk fibroin was recently demonstrated starting from methacrylated silk fibroin and choosing a protein as template. Here, we attempted the imprinting of fibroin-based molecularly imprinted polymers (MIPs), called bioMIPs, using as a template hepcidin that is a iron-metabolism regulator-peptide, possessing a hairpin structure. A homogeneous population (PDI < 0.2) of bioMIPs with size ~50 nm was produced. The bioMIPs were selective for the template; the estimated dissociation constant for hepcidin was K = 3.6 ± 0.5 10 M and the average number of binding sites per bioMIP was equal to 2. The bioMIPs used in a competitive assay for hepcidin in serum showed a detection range of 1.01 10- 6.82 10 M and a limit of detection of 3.29 10 M.
最近有人证明，通过甲基丙烯酰化丝素蛋白并选择一种蛋白质作为模板，从丝素中制备分子印迹纳米粒子是可行的。在这里，我们尝试使用铁代谢调节剂肽 - 具有发夹结构的铁调素作为模板来印迹基于丝素的分子印迹聚合物（MIPs），称为生物 MIPs。制备了尺寸约为 50nm 的均匀生物 MIPs （PDI <0.2）。生物 MIPs 对模板具有选择性; 铁调素的估计解离常数为 K = 3.6 ± 0.5 10 M，每个生物 MIP 的结合位点平均数等于 2。在用于检测血清中铁调素的竞争性测定中，生物 MIPs 的检测范围为 1.01 10-6.82 10 M，检测限为 3.29 10 M。
