Dwijayanti Ari, Zhang Congqiang, Poh Chueh Loo, Lautier Thomas
CNRS@CREATE, Singapore, Singapore.
Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (ASTAR), Singapore, Singapore.
Front Bioeng Biotechnol. 2022 Jan 5;9:804563. doi: 10.3389/fbioe.2021.804563. eCollection 2021.
Owing to its ubiquity and easy availability in nature, light has been widely employed to control complex cellular behaviors. Light-sensitive proteins are the foundation to such diverse and multilevel adaptive regulations in a large range of organisms. Due to their remarkable properties and potential applications in engineered systems, exploration and engineering of natural light-sensitive proteins have significantly contributed to expand optogenetic toolboxes with tailor-made performances in synthetic genetic circuits. Progressively, more complex systems have been designed in which multiple photoreceptors, each sensing its dedicated wavelength, are combined to simultaneously coordinate cellular responses in a single cell. In this review, we highlight recent works and challenges on multiplexed optogenetic circuits in natural and engineered systems for a dynamic regulation breakthrough in biotechnological applications.
由于光在自然界中无处不在且易于获取，它已被广泛用于控制复杂的细胞行为。光敏感蛋白是众多生物中这种多样且多层次适应性调节的基础。由于其卓越的特性以及在工程系统中的潜在应用，对天然光敏感蛋白的探索和工程改造极大地推动了光遗传学工具箱的扩展，使其在合成遗传电路中具有定制的性能。逐渐地，人们设计出了更复杂的系统，其中多个各自感应特定波长的光感受器被组合起来，以在单个细胞中同时协调细胞反应。在这篇综述中，我们重点介绍了天然和工程系统中用于生物技术应用动态调节突破的多重光遗传学电路的最新研究成果和挑战。
