Suppr超能文献

在大肠杆菌K-12的serC突变体中,3-羟基丙酮酸可替代吡哆醇。

3-hydroxypyruvate substitutes for pyridoxine in serC mutants of Escherichia coli K-12.

作者信息

Shimizu S, Dempsey W B

出版信息

J Bacteriol. 1978 Jun;134(3):944-9. doi: 10.1128/jb.134.3.944-949.1978.

Abstract

Escherichia coli K-12 mutants with serC genotype required pyridoxine and serine for normal growth, as do E. coli B mutants of this type. Mutants of the K-12 strain, however, reverted easily to pyridoxine independence without regaining activity in the 3-phosphoserine oxoglutarate transaminase coded for by the serC gene. Both these revertants and the parental type synthesized pyridoxine in normal amounts when 3-hydroxypyruvate was used as a supplement, although neither of these mutants could use this compound to satisfy their serine requirement. Since serine alone was inadequate to provide the nutritional requirement of serC mutants, these mutants must have been unable to synthesize 3-hydroxypyruvate from serine. We suggest that 3-phosphoserine oxoglutarate transaminase in normal E. coli serves as a catalyst for transaminating small amounts of serine to 3-hydroxypyruvate, which is then used in pyridoxine biosynthesis. In serC mutants, this activity is blocked, and these mutants then show a double requirement for serine and pyridoxine.

摘要

具有serC基因型的大肠杆菌K - 12突变体正常生长需要吡哆醇和丝氨酸,这种类型的大肠杆菌B突变体也是如此。然而,K - 12菌株的突变体很容易回复到对吡哆醇不依赖的状态,而serC基因编码的3 - 磷酸丝氨酸酮戊二酸转氨酶却没有恢复活性。当使用3 - 羟基丙酮酸作为补充剂时,这些回复突变体和亲本类型都能正常合成吡哆醇,尽管这些突变体都不能利用这种化合物来满足其丝氨酸需求。由于仅丝氨酸不足以满足serC突变体的营养需求,这些突变体一定无法从丝氨酸合成3 - 羟基丙酮酸。我们认为,正常大肠杆菌中的3 - 磷酸丝氨酸酮戊二酸转氨酶作为一种催化剂,将少量丝氨酸转氨生成3 - 羟基丙酮酸,然后用于吡哆醇的生物合成。在serC突变体中,这种活性被阻断,这些突变体随后表现出对丝氨酸和吡哆醇的双重需求。

相似文献

1
3-hydroxypyruvate substitutes for pyridoxine in serC mutants of Escherichia coli K-12.
J Bacteriol. 1978 Jun;134(3):944-9. doi: 10.1128/jb.134.3.944-949.1978.
2
Metabolic relationships between pyridoxine (vitamin B6) and serine biosynthesis in Escherichia coli K-12.
J Bacteriol. 1990 Nov;172(11):6518-28. doi: 10.1128/jb.172.11.6518-6528.1990.
3
Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants.
J Bacteriol. 1970 Nov;104(2):658-67. doi: 10.1128/jb.104.2.658-667.1970.
4
3-Phosphoserine transaminase mutants of Escherichia coli B.
J Bacteriol. 1969 Nov;100(2):1114-5. doi: 10.1128/jb.100.2.1114-1115.1969.
5
4-O-phosphoryl-L-threonine, a substrate of the pdxC(serC) gene product involved in vitamin B6 biosynthesis.
FEBS Lett. 1996 Jul 22;390(2):179-82. doi: 10.1016/0014-5793(96)00652-7.
7
Regulation of septation: a novel role for SerC/PdxF in Salmonella?
Mol Gen Genet. 2000 Sep;264(1-2):184-92. doi: 10.1007/s004380000311.
8
Biosynthesis of vitamin B 6 .
J Biol Chem. 1972 Mar 25;247(6):1869-82.
9
Biosynthesis of pyridoxol in Escherichia coli.
Nutr Rev. 1972 Oct;30(10):238-40. doi: 10.1111/j.1753-4887.1972.tb03982.x.

引用本文的文献

1
Engineering and finetuning expression of SerC for balanced metabolic flux in vitamin B production.
Synth Syst Biotechnol. 2024 Mar 20;9(2):388-398. doi: 10.1016/j.synbio.2024.03.005. eCollection 2024 Jun.
2
Organ agar serves as physiologically relevant alternative for bacterial colonization.
Infect Immun. 2023 Nov 16;91(11):e0035523. doi: 10.1128/iai.00355-23. Epub 2023 Oct 18.
4
Global map of growth-regulated gene expression in Burkholderia pseudomallei, the causative agent of melioidosis.
J Bacteriol. 2006 Dec;188(23):8178-88. doi: 10.1128/JB.01006-06. Epub 2006 Sep 22.
5
Linkage map of Escherichia coli K-12, edition 10: the traditional map.
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
6
Linkage map of Escherichia coli K-12, edition 6.
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
10
Genetic characterization of a highly efficient alternate pathway of serine biosynthesis in Escherichia coli.
J Bacteriol. 1987 Jun;169(6):2611-7. doi: 10.1128/jb.169.6.2611-2617.1987.

本文引用的文献

1
CONTROL OF PYRIDOXINE BIOSYNTHESIS IN ESCHERICHIA COLI.
J Bacteriol. 1965 Aug;90(2):431-7. doi: 10.1128/jb.90.2.431-437.1965.
2
NUTRITIONAL AND REGULATORY ASPECTS OF SERINE METABOLISM IN ESCHERICHIA COLI.
J Bacteriol. 1964 Sep;88(3):611-9. doi: 10.1128/jb.88.3.611-619.1964.
4
The synthesis of vitamin B6 by some mutant strains of Escherichia coli.
J Gen Microbiol. 1959 Jun;20(3):597-604. doi: 10.1099/00221287-20-3-597.
5
Inter-relationships of serine, glycine and vitamin B6 in the growth of mutants of Escherichia coli.
J Gen Microbiol. 1959 Jun;20(3):576-96. doi: 10.1099/00221287-20-3-576.
6
3-Phosphoserine transaminase mutants of Escherichia coli B.
J Bacteriol. 1969 Nov;100(2):1114-5. doi: 10.1128/jb.100.2.1114-1115.1969.
7
Characterization of pyridoxine auxotrophs of Escherichia coli: P1 transduction.
J Bacteriol. 1969 Mar;97(3):1403-10. doi: 10.1128/jb.97.3.1403-1410.1969.
10
Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants.
J Bacteriol. 1970 Nov;104(2):658-67. doi: 10.1128/jb.104.2.658-667.1970.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验