Grenier Vincent, Martinez Kayli N, Benlian Brittany R, García-Almedina Derek M, Raliski Benjamin K, Boggess Steven C, Maza Johnathan C, Yang Samantha J, Gest Anneliese M M, Miller Evan W
Department of Chemistry and Department of Molecular and Cell Biology and Helen Wills Neuroscience Institute. University of California, Berkeley, California 94720, United States.
ACS Cent Sci. 2022 Jan 26;8(1):118-121. doi: 10.1021/acscentsci.1c01153. Epub 2022 Jan 13.
Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes.
电压敏感荧光报告分子可以揭示神经元和心肌细胞中膜电位的快速变化。然而，在许多情况下，荧光报告分子存在时的光照会导致动作电位形状的破坏，从而限制记录时间。我们在此表明，一种以前仅限于荧光团而非指示剂的分子修复方法可用于大幅延长对神经元和心肌细胞的成像时间。
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