Functional verification and screening of protein interacting with the slPHB3.

was cloned from , the amino acid sequence blast and phylogenetic tree analysis showed that slPHB3 has the most similarity with PHB3 from using DNAMAN software and MEGA7 software. RT-qPCR results confirmed that the expression of was induced obviously under stress treatments. The growth of recombinant yeast cells was better than that of the control group under the stress treatment, indicating that may be involved in the stress response of yeast cells. The transgenic tobacco was treated with different concentrations of NaCl, NaHCO and HO, fresh weigh of overexpression tobacco were heavier than wild-types. The results showed that transgenic tobacco was more tolerant to salt and oxidation than wild-type tobacco. Expression of important genes including and in salt stress response pathways are steadily higher in overexpression tobacco than that in wild-types. We identified 17 proteins interacting with slPHB3 by yeast two-hybrid technique, most of these proteins were relation to the stresses. The salt tolerance of expressing yeast and overexpressing plants were better than that of the control. Ten stress-related proteins may interact with slPHB3, which preliminarily indicated that had a certain response relationship with salt stress. The study of under abiotic stress can improve our understanding of gene function.