Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, China.
College of Forestry, Northeast Forestry University, Harbin, China.
Plant Signal Behav. 2022 Dec 31;17(1):2025678. doi: 10.1080/15592324.2022.2025678. Epub 2022 Feb 3.
was cloned from , the amino acid sequence blast and phylogenetic tree analysis showed that slPHB3 has the most similarity with PHB3 from using DNAMAN software and MEGA7 software. RT-qPCR results confirmed that the expression of was induced obviously under stress treatments. The growth of recombinant yeast cells was better than that of the control group under the stress treatment, indicating that may be involved in the stress response of yeast cells. The transgenic tobacco was treated with different concentrations of NaCl, NaHCO and HO, fresh weigh of overexpression tobacco were heavier than wild-types. The results showed that transgenic tobacco was more tolerant to salt and oxidation than wild-type tobacco. Expression of important genes including and in salt stress response pathways are steadily higher in overexpression tobacco than that in wild-types. We identified 17 proteins interacting with slPHB3 by yeast two-hybrid technique, most of these proteins were relation to the stresses. The salt tolerance of expressing yeast and overexpressing plants were better than that of the control. Ten stress-related proteins may interact with slPHB3, which preliminarily indicated that had a certain response relationship with salt stress. The study of under abiotic stress can improve our understanding of gene function.
该基因是从 中克隆得到的，氨基酸序列比对和系统进化树分析表明，slPHB3 与 来源的 PHB3 同源性最高。利用 DNAMAN 软件和 MEGA7 软件进行氨基酸序列比对和系统进化树分析，结果表明 slPHB3 与 来源的 PHB3 同源性最高。RT-qPCR 结果证实，在胁迫处理下 slPHB3 的表达明显受到诱导。在胁迫处理下，重组酵母细胞的生长优于对照组，表明 slPHB3 可能参与酵母细胞的应激反应。将转基因烟草分别用不同浓度的 NaCl、NaHCO 和 HO 处理，过表达烟草的鲜重比野生型重。结果表明，转基因烟草对盐和氧化胁迫的耐受性强于野生型烟草。在盐胁迫应答途径中，包括 和 在内的重要基因的表达在过表达烟草中比野生型烟草稳定更高。通过酵母双杂交技术鉴定到与 slPHB3 相互作用的 17 种蛋白质，这些蛋白质大多数与应激有关。表达 slPHB3 的酵母和过表达植株的耐盐性均优于对照。10 种与应激相关的蛋白质可能与 slPHB3 相互作用，这初步表明 slPHB3 与盐胁迫有一定的应答关系。对非生物胁迫下 slPHB3 的研究可以提高我们对 slPHB3 基因功能的理解。
