Yang Endong, Zhao Meiyi, Li Shanshan, Wang Yanxiang, Sun Leni, Liu Jing, Wang Weiyun
School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
Int J Syst Evol Microbiol. 2019 Jul;69(7):2135-2141. doi: 10.1099/ijsem.0.003449. Epub 2019 May 29.
A bacterial strain, designated SOD, with Gram-stain-negative and motile rod-shaped cells, was isolated from soil in Hefei, PR China, and was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SOD belonged to the genus Massilia and showed the highest similarities to Massilia violaceinigra B2 (99.3 %), followed by Massilia glaciei B448-2 (98.7 %), Massilia eurypsychrophila CGMCC 1.12828 (98.6 %) and Rugamonas rubra CCM3730 (97.8 %). Average nucleotide identity and digital DNA-DNA hybridization values between genome sequences of strain SOD and the closely related species ranged from 77.1 to 89.3% and from 22.2 to 34.7 %. The DNA G+C content of strain SOD was 65.4 mol%. Strain SOD contained Q-8 as the major ubiquinone and the dominant fatty acids were summed feature 3 (C16 : 1ω7c and/or C15 : 0iso 2-OH; 58.5 %), C16 : 0 (26.8 %) and C18 : 1ω7c (5.0 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. On the basis of the evidence presented in this study, strain SOD represents a novel species of the genus Massilia, for which the name Massiliaatriviolacea sp. nov. is proposed. The type strain is SOD (=KCTC 62720=LMG 30840).
从中国合肥土壤中分离出一株革兰氏阴性、具运动性的杆状细菌，命名为SOD，并采用多相分类法对其进行了表征。基于16S rRNA基因序列的系统发育分析表明，菌株SOD属于马赛菌属，与紫黑马赛菌B2相似度最高（99.3%），其次是冰川马赛菌B448-2（98.7%）、嗜广温马赛菌CGMCC 1.12828（98.6%）和红色鲁加莫纳斯菌CCM3730（97.8%）。菌株SOD与密切相关物种的基因组序列之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为77.1%至89.3%和22.2%至34.7%。菌株SOD的DNA G+C含量为65.4 mol%。菌株SOD以泛醌Q-8作为主要醌类，主要脂肪酸为总和特征3（C16:1ω7c和/或C15:0iso 2-OH；58.5%）、C16:0（26.8%）和C18:1ω7c（5.0%）。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。根据本研究提供的证据，菌株SOD代表马赛菌属的一个新物种，为此提出新名称紫黑马赛菌（Massilia atriviolacea sp. nov.）。模式菌株为SOD（=KCTC 62720=LMG 30840）。
