Massilia atriviolacea sp. nov., a dark purple-pigmented bacterium isolated from soil.

A bacterial strain, designated SOD, with Gram-stain-negative and motile rod-shaped cells, was isolated from soil in Hefei, PR China, and was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SOD belonged to the genus Massilia and showed the highest similarities to Massilia violaceinigra B2 (99.3 %), followed by Massilia glaciei B448-2 (98.7 %), Massilia eurypsychrophila CGMCC 1.12828 (98.6 %) and Rugamonas rubra CCM3730 (97.8 %). Average nucleotide identity and digital DNA-DNA hybridization values between genome sequences of strain SOD and the closely related species ranged from 77.1 to 89.3% and from 22.2 to 34.7 %. The DNA G+C content of strain SOD was 65.4 mol%. Strain SOD contained Q-8 as the major ubiquinone and the dominant fatty acids were summed feature 3 (C16 : 1ω7c and/or C15 : 0iso 2-OH; 58.5 %), C16 : 0 (26.8 %) and C18 : 1ω7c (5.0 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. On the basis of the evidence presented in this study, strain SOD represents a novel species of the genus Massilia, for which the name Massiliaatriviolacea sp. nov. is proposed. The type strain is SOD (=KCTC 62720=LMG 30840).