He Jia-Peng, Tian Qing, Zhu Qiu-Yang, Liu Ji-Long
Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Tianhe District, No. 483 Wushan Road, Guangzhou, 510642, China.
Cell Biosci. 2022 Feb 5;12(1):13. doi: 10.1186/s13578-022-00749-y.
Embryo implantation into the uterus is a crucial step for human reproduction. A hypothesis has been proposed that the molecular circuit invented by trophoblasts for invasive embryo implantation during evolution might be misused by cancer cells to promote malignancy. Unfortunately, our current understanding of the molecular mechanism underlying embryo implantation is far from complete.
Here we used the mouse as an animal model and generated a single-cell transcriptomic atlas of the embryo implantation site of mouse uterus at the invasion phase of embryo implantation on gestational day 6. We revealed 23 distinct cell clusters, including 5 stromal cell clusters, 2 epithelial cell clusters, 1 smooth muscle cell cluster, 2 pericyte clusters, 4 endothelial cell clusters, and 9 immune cell clusters. Through data analysis, we identified differentially expression changes in all uterine cell types upon embryo implantation. By integrated with single-cell RNA-seq data from E5.5 embryos, we predicted cell-cell crosstalk between trophoblasts and uterine cell types.
Our study provides a valuable resource for understanding of the molecular mechanism of embryo implantation.
胚胎植入子宫是人类生殖的关键步骤。有假说提出，滋养层细胞在进化过程中为侵袭性胚胎植入所发明的分子回路可能被癌细胞滥用，从而促进恶性肿瘤的发展。不幸的是，我们目前对胚胎植入潜在分子机制的理解还远远不够完善。
在此，我们以小鼠为动物模型，构建了妊娠第6天胚胎植入侵袭阶段小鼠子宫胚胎植入部位的单细胞转录组图谱。我们揭示了23个不同的细胞簇，包括5个基质细胞簇、2个上皮细胞簇、1个平滑肌细胞簇、2个周细胞簇、4个内皮细胞簇和9个免疫细胞簇。通过数据分析，我们确定了胚胎植入后所有子宫细胞类型的差异表达变化。通过整合来自E5.5胚胎的单细胞RNA测序数据，我们预测了滋养层细胞与子宫细胞类型之间的细胞间串扰。
我们的研究为理解胚胎植入的分子机制提供了宝贵资源。
