Universidade da Coruña, Tissue Engineering and Cellular Therapy Group, Instituto de Investigación Biomédica de A Coruña (INIBIC), Centro de Investigaciones Científicas Avanzadas (CICA), 15006 A Coruña, Spain.
CINBIO, Universidade de Vigo, Biomass and Sustanaible Development Group (EQ2), Departament of Chemical Engineering, 32004 Ourense, Spain.
Carbohydr Polym. 2022 Apr 15;282:119134. doi: 10.1016/j.carbpol.2022.119134. Epub 2022 Jan 12.
Synovial fibrosis is a pathological process which contributes to joint pain and stiffness in several musculoskeletal disorders. Fucoidans, sulfated polysaccharides found in brown algae, have recently emerged as promising therapeutic agents. Despite the increasing amount of evidence suggesting the protective role of fucoidans in different experimental approaches of human fibrotic disorders, the effect of these sulfated polysaccharides on synovial fibrosis has not been investigated yet. By an in vitro experimental approach in fibroblast-like synoviocytes, we detected that fucoidans inhibit their differentiation into myofibroblasts with tumor cell-like characteristics and restore apoptosis. Composition and structure of fucoidan appear to be critical for the detected activity. Furthermore, protective effects of these sulfated polysaccharides are mediated by upregulation of nitric oxide production and modulation of TGF-β/smad pathway. Altogether, our results support the use of fucoidans as therapeutic compounds in the treatment of the fibrotic processes involved in rheumatic pathologies.
滑液纤维组织增生是一种病理过程，会导致几种肌肉骨骼疾病的关节疼痛和僵硬。褐藻中发现的硫酸多糖岩藻聚糖，最近成为很有前途的治疗药物。尽管越来越多的证据表明岩藻聚糖在人类纤维性疾病的不同实验方法中具有保护作用，但这些硫酸多糖对滑液纤维组织增生的影响尚未得到研究。通过成纤维细胞样滑膜细胞的体外实验方法，我们发现岩藻聚糖抑制其分化为具有肿瘤细胞样特征的肌成纤维细胞，并恢复细胞凋亡。岩藻聚糖的组成和结构似乎对检测到的活性很关键。此外，这些硫酸多糖的保护作用是通过上调一氧化氮的产生和调节 TGF-β/smad 途径来介导的。总之，我们的结果支持将岩藻聚糖用作治疗风湿性疾病中涉及的纤维性过程的治疗化合物。
