Nair J, Ohshima H, Malaveille C, Friesen M, O'Neill I K, Hautefeuille A, Bartsch H
Food Chem Toxicol. 1986 Jan;24(1):27-31. doi: 10.1016/0278-6915(86)90260-7.
During N-nitrosamine analysis of extracts of betel quid with tobacco and of the saliva of chewers of betel quid with tobacco for N-nitrosamines using a Thermal Energy Analyzer, two unknown compounds were detected. They were identified as synthetic nitro musks, musk ambrette (5-tert-butyl-1,3-dinitro-4-methoxy-2-methylbenzene, CAS No. 83-66-9) and musk xylene, (1-tert-butyl-3,5-dimethyl-2,4,6-trinitrobenzene, CAS No. 81-15-2), by gas chromatography-mass spectrometry and Fourier transform nuclear magnetic resonance spectroscopy. These compounds were detected in several samples of betel quid with tobacco and in perfumed tobacco used for chewing in India in amounts ranging from 0.45-23.5 mg/g wet weight. Musk ambrette was found to be mutagenic in Salmonella typhimurium TA100 requiring metabolic activation by rat-liver postmitochondrial supernatant but musk xylene lacked mutagenicity.
在使用热能分析仪对含烟草的槟榔咀嚼物提取物以及含烟草的槟榔咀嚼者的唾液进行N-亚硝胺分析时，检测到了两种未知化合物。通过气相色谱-质谱联用仪和傅里叶变换核磁共振光谱法，它们被鉴定为合成硝基麝香，即葵子麝香（5-叔丁基-1,3-二硝基-4-甲氧基-2-甲基苯，CAS编号83-66-9）和二甲苯麝香（1-叔丁基-3,5-二甲基-2,4,6-三硝基苯，CAS编号81-15-2）。这些化合物在印度的多个含烟草的槟榔咀嚼物样本以及用于咀嚼的加香烟草中被检测到，含量范围为0.45 - 23.5毫克/克湿重。葵子麝香在鼠伤寒沙门氏菌TA100中具有致突变性，需要经大鼠肝脏线粒体后上清液进行代谢激活，但二甲苯麝香没有致突变性。
