Two Alkaloids From Royle, Their Anti-inflammatory and Anti-oxidative Stress Activity NF-κB Signaling Pathway.

In this study, we isolated and identified four compounds in Royle, and they were Delbrunine (), 4--α-D-Glucosyl benzoic acid (), Kaempferol 3--β-D-glucopyranoside 7--α-L-rhamnopyranoside () and Eldeline (). Furthermore, the anti-inflammatory activity of these compounds was screened in RAW264.7 cells. The results showed that the anti-inflammatory activities of compounds and were weak, and , had good anti-inflammatory activity. The macrophage inflammation model was established by lipopolysaccharide (LPS). Then, the anti-inflammatory activity was evaluated by ELISA kits, qRT-PCR experiment and western blot experiment. And the anti-oxidative stress activity was assessed by flow cytometry. The results showed that compounds , could significantly inhibit the elevation of inflammatory factors nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and also had obvious inhibitory effects on the production of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). In addition, compounds and could effectively inhibit the overexpression of reactive oxygen species (ROS) in RAW264.7 cells that activated by LPS. These results indicated that compounds and may exert anti-inflammatory and anti-oxidative stress effects through the NF-κB signaling pathway.