使用生物发光致病细菌对抗菌制剂进行快速评估
Rapid and Evaluation of Antimicrobial Formulations Using Bioluminescent Pathogenic Bacteria.
作者信息
Schmidtchen Artur, Puthia Manoj
机构信息
Division of Dermatology and Venereology, Department of Clinical Sciences, Lund University, SE-22184 Lund, Sweden.
Copenhagen Wound Healing Center, Bispebjerg Hospital, Department of Biomedical Sciences, University of Copenhagen, DK-2400, Denmark.
出版信息
Bio Protoc. 2022 Jan 20;12(2):e4302. doi: 10.21769/BioProtoc.4302.
Basic and translational research needs rapid methods to test antimicrobial formulations. Bioluminescent bacteria and advanced imaging systems capable of acquiring bioluminescence enable us to quickly and longitudinally evaluate the efficacy of antimicrobials. Conventional approaches, such as radial diffusion and viable count assays, are time-consuming and do not allow for longitudinal analysis. Bioluminescence imaging is sensitive and gives vital spatial and temporal information on the infection status in the body. Here, using bioluminescent , we describe an and an approach to rapidly evaluate the antimicrobial efficacy of the host-defense peptide TCP-25. Graphic abstract: Evaluation of antimicrobials using bioluminescent bacteria.
基础研究和转化研究需要快速的方法来测试抗菌制剂。发光细菌和能够获取生物发光的先进成像系统使我们能够快速且纵向地评估抗菌药物的疗效。传统方法，如径向扩散法和活菌计数法，耗时且无法进行纵向分析。生物发光成像灵敏，能提供关于体内感染状态的重要时空信息。在此，我们利用发光细菌描述了一种快速评估宿主防御肽TCP - 25抗菌疗效的体内和体外方法。图形摘要：使用发光细菌评估抗菌药物。
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