Yoon Jaewoo
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu, 42601, Republic of Korea.
Antonie Van Leeuwenhoek. 2022 Apr;115(4):487-495. doi: 10.1007/s10482-022-01711-1. Epub 2022 Feb 7.
A novel gammaproteobacterium, designated as KMU-158, was isolated from seawater collected on the coastline of Dadaepo in the Republic of Korea, and characterized using a polyphasic taxonomic approach. Strain KMU-158 was Gram-staining-negative, pale beige-colored, strictly aerobic, rod-shaped, motile, and chemoorganoheterotrophic. The novel isolate was able to grow at 0-3% NaCl concentrations (w/v), pH 6.5-9.5, and 15-40 °C. The analysis based on 16S rRNA gene sequences indicated that strain KMU-158 belongs to the family Spongiibacteraceae and shared the highest similarity with Spongiibacter tropicus CL-CB221 (96.1%). The major cellular fatty acids were summed feature 3 (C ω7c and/or C ω6c) and C ω8c. The only respiratory quinone was identified as ubiquinone-8. The polar lipids of strain KMU-158 contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and two unidentified lipids. The assembled draft genome size of strain KMU-158 was 3.29 Mbp with a DNA G + C content of 51.3%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values of KMU-158 and the representatives of the genus Spongiibacter were found to be 78.5-79.1%, 13.8-14.1%, and 66.6-66.8%, respectively. From the distinct phenotypic, phylogenetic, genomic, and chemotaxonomic properties, the strain KMU-158 is considered to represent a novel species of the genus Spongiibacter, for which the name Spongiibacter pelagi sp. nov. is proposed. The type strain of the species S. pelagi sp. nov. is KMU-158 (= KCCM 90448 = NBRC 114307).
从韩国巨济岛海岸线采集的海水中分离出一株新型γ-变形菌，命名为KMU-158，并采用多相分类方法对其进行了表征。菌株KMU-158革兰氏染色阴性，浅米色，严格需氧，杆状，具运动性，属于化能有机异养型。该新型菌株能够在0-3%（w/v）的NaCl浓度、pH值6.5-9.5和15-40℃的条件下生长。基于16S rRNA基因序列的分析表明，菌株KMU-158属于海绵杆菌科，与热带海绵杆菌CL-CB221的相似度最高（96.1%）。主要细胞脂肪酸为特征性总和3（C ω7c和/或C ω6c）和C ω8c。唯一的呼吸醌被鉴定为泛醌-8。菌株KMU-158的极性脂质包含磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油和两种未鉴定的脂质。菌株KMU-158的组装草图基因组大小为3.29 Mbp，DNA G+C含量为51.3%。发现KMU-158与海绵杆菌属代表菌株的平均核苷酸同一性、数字DNA-DNA杂交和平均氨基酸同一性值分别为78.5-79.1%、13.8-14.1%和66.6-66.8%。基于独特的表型、系统发育、基因组和化学分类学特性，菌株KMU-158被认为代表海绵杆菌属的一个新物种，为此提出了新名称海绵海杆菌（Spongiibacter pelagi sp. nov.）。海绵海杆菌新物种的模式菌株是KMU-158（=KCCM 90448=NBRC 114307）。
