College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu, 42601, Republic of Korea.
Arch Microbiol. 2022 Apr 21;204(5):273. doi: 10.1007/s00203-022-02888-9.
A bacterium, designated as KMU-166, belonging to the class Gammaproteobacteria, was isolated from seawater collected on the coastline of Dadaepo, Republic of Korea. Strain KMU-166 was Gram-staining-negative, ovoid-shaped, motile, strictly aerobic, beige-colored, catalase-positive, and oxidase-negative; and had a chemoorganoheterotrophic metabolism. The novel isolate was found to grow at 1-4% NaCl concentrations (w/v), pH 6.5-9.5, and 10-40 °C. The 16S rRNA gene sequence-based phylogeny showed that strain KMU-166 affiliates to the family Spongiibacteraceae and that it shared the greatest sequence similarity (96.4%) with Spongiibacter marinus HAL40b. The main (> 10%) cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c), C17:1ω8c, and C18:1ω7c. The predominant respiratory quinone was ubiquinone-8 (Q-8). Strain KMU-166 comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and two unidentified lipids. The assembled draft genome was 4.40 Mbp in size with a DNA G+C content of 55.7%. The average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values of KMU-166 and Spongiibacter marinus HAL40b, Spongiibacter tropicus CL-CB221, and "Spongiibacter pelagi" KMU-158 were found to be 77.7-79.8%, 13.7-15.4%, and 66.1-70.9%, respectively. From the distinguishable polyphasic taxonomic results obtained, the strain is considered to represent a novel species of the genus Spongiibacter for which the name Spongiibacter thalassae sp. nov. is proposed. The type strain of S. thalassae sp. nov. is KMU-166 (= KCCM 90449 = NBRC 114308).
一株细菌，命名为 KMU-166，属于γ变形菌纲，是从韩国大浦海岸采集的海水中分离出来的。菌株 KMU-166 革兰氏染色阴性，呈卵形，运动性，严格需氧，米色，过氧化氢酶阳性，氧化酶阴性；具有化能有机异养代谢。新分离株在 1-4%（w/v）NaCl 浓度、pH 值 6.5-9.5 和 10-40°C 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 KMU-166 属于海绵杆菌科，与海绵杆菌 HAL40b 的序列相似性最高（96.4%）。主要（>10%）细胞脂肪酸为 C16:1ω7c 和/或 C16:1ω6c、C17:1ω8c 和 C18:1ω7c。主要呼吸醌为泛醌-8（Q-8）。菌株 KMU-166 由磷脂酰乙醇胺、磷脂酰甘油、双磷脂酰甘油和两种未鉴定的脂质组成。组装的基因组草图大小为 4.40 Mbp，GC 含量为 55.7%。KMU-166 和海绵杆菌 HAL40b、海绵杆菌 tropicus CL-CB221 和“海绵杆菌 pelagi”KMU-158 的平均核苷酸同一性、数字 DNA-DNA 杂交和平均氨基酸同一性值分别为 77.7-79.8%、13.7-15.4%和 66.1-70.9%。从可区分的多相分类学结果来看，该菌株被认为代表了海绵杆菌属的一个新种，命名为海绵杆菌海洋亚种。新种的模式菌株为 KMU-166（=KCCM 90449=NBRC 114308）。
