Machida I, Nakai S
Mutat Res. 1980 Nov;73(1):59-68. doi: 10.1016/0027-5107(80)90135-9.
Inductions of reversions of nonsense, missense and frameshift-type mutations were investigated in a diploid cell population of Saccharomyces cerevisiae during commitment to meiosis, by using the medium-transfer technique from sporulation medium to vegetative medium. The yields of spontaneous reverse mutations obtained from the cells that were committed to different stages during meiosis were rather constant irrespective of the alleles tested, although the yields of both intergenic and intragenic recombinations markedly increased. The susceptibilities to UV-induced reverse mutations examined during commitment to meiosis were not changed appreciably. It is concluded that induction of base-change-type mutations in meiosis is not essentially different from that in mitosis.
通过使用从孢子形成培养基转移到营养培养基的培养基转移技术，在酿酒酵母的二倍体细胞群体进入减数分裂过程中，研究了无义、错义及移码型突变回复的诱导情况。从进入减数分裂不同阶段的细胞中获得的自发回复突变率，无论所测试的等位基因如何，都相当恒定，尽管基因间和基因内重组率都显著增加。在进入减数分裂过程中检测的对紫外线诱导回复突变的敏感性没有明显变化。得出的结论是，减数分裂中碱基变化型突变的诱导与有丝分裂中的诱导没有本质区别。
