Institute of Biology, University of Hohenheim, Stuttgart, Germany; email:
Annu Rev Plant Biol. 2022 May 20;73:173-200. doi: 10.1146/annurev-arplant-102720-022810. Epub 2022 Feb 7.
The advent of high-throughput sequencing-based methods for chromatin conformation, accessibility, and immunoprecipitation assays has been a turning point in 3D genomics. Altogether, these new tools have been pushing upward the interpretation of pioneer cytogenetic evidence for a higher order in chromatin packing. Here, we review the latest development in our understanding of plant spatial genome structures and different levels of organization and discuss their functional implications. Then, we spotlight the complexity of organellar (i.e., mitochondria and plastids) genomes and discuss their 3D packing into nucleoids. Finally, we propose unaddressed research axes to investigate functional links between chromatin-like dynamics and transcriptional regulation within organellar nucleoids.
基于高通量测序的染色质构象、可及性和免疫沉淀分析方法的出现，是三维基因组学的一个转折点。总的来说，这些新工具推动了对染色质包装更高阶的先驱细胞遗传学证据的解释。在这里，我们回顾了我们对植物空间基因组结构和不同层次组织的最新理解，并讨论了它们的功能意义。然后，我们强调了细胞器（即线粒体和质体）基因组的复杂性，并讨论了它们三维包装成核区。最后，我们提出了未解决的研究方向，以研究细胞器核区中类似染色质的动力学与转录调控之间的功能联系。
