Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Bioengineered. 2022 Feb;13(2):2428-2439. doi: 10.1080/21655979.2021.2011639.
Recently, various studies have suggested that circular RNAs (circRNAs) are ubiquitous in various malignant events, including non-small cell lung cancer (NSCLC) and are closely related to cell proliferation and apoptosis. Unfortunately, the molecular functions involved in this action still have little overlap. Therefore, this study aimed to identify a novel circCAMSAP1 role in NSCLC. Overexpression of circCAMSAP1 has been demonstrated in NSCLC lung tissues and cell lines. Sequencing and RNase R experiments were planned to determine whether circCAMSAP1 is looped and exists in NSCLC. We also found that downregulated circCAMSAP1 repressed cell proliferation and increased apoptosis of NSCLC cells and suppressed xenograft tumor growth . Furthermore, a luciferase assay revealed that circCAMSAP1 could regulate baculoviral inhibitor of apoptosis protein (IAP) repeat containing 5 (BIRC5, also known as survivin) expression by directly binding to miR-1182. However, BIRC5 without 3' untranslated regions (3'UTR) could reverse the influence of downregulated circCAMSAP1 on proliferation and apoptosis in NSCLC. Together, our findings reveal a novel mechanism by which the circCAMSAP1/miR-1182/BIRC5 axis promotes NSCLC progression.
最近，各种研究表明，环状 RNA（circRNA）在各种恶性事件中普遍存在，包括非小细胞肺癌（NSCLC），并且与细胞增殖和凋亡密切相关。不幸的是，涉及此作用的分子功能仍然很少重叠。因此，本研究旨在鉴定 circCAMSAP1 在 NSCLC 中的新作用。circCAMSAP1 在 NSCLC 肺组织和细胞系中的表达增加。计划进行测序和 RNase R 实验以确定 circCAMSAP1 是否是环状的并且存在于 NSCLC 中。我们还发现，下调的 circCAMSAP1 抑制 NSCLC 细胞的增殖和增加凋亡，并抑制异种移植肿瘤的生长。此外，荧光素酶测定表明，circCAMSAP1 可以通过直接结合 miR-1182 来调节杆状病毒凋亡蛋白抑制因子（IAP）重复序列 5（BIRC5，也称为生存素）的表达。然而，没有 3'非翻译区（3'UTR）的 BIRC5 可以逆转下调 circCAMSAP1 对 NSCLC 增殖和凋亡的影响。总之，我们的研究结果揭示了 circCAMSAP1/miR-1182/BIRC5 轴促进 NSCLC 进展的新机制。
