Hamlyn Centre, Imperial College London, London, SW7 2AZ, UK.
Department of Brain Science, Imperial College London, London, W12 0BZ, UK; Care Research & Technology Centre, UK Dementia Research Institute, London, W12 0BZ, UK.
Biosens Bioelectron. 2022 May 1;203:114050. doi: 10.1016/j.bios.2022.114050. Epub 2022 Jan 29.
Accurate, reliable, and cost-effective immunosensors are clinically important for the early diagnosis and monitoring of progressive diseases, and multiplexed sensing is a promising strategy for the next generation of diagnostics. This strategy allows for the simultaneous detection and quantification of multiple biomarkers with significantly enhanced reproducibility and reliability, whilst requiring smaller sample volumes, fewer materials, and shorter average analysis time for individual biomarkers than individual tests. In this opinionated review, we compare different techniques for the development of multiplexed immunosensors. We review the state-of-the-art approaches in the field of multiplexed immunosensors using electrical, electrochemical, and optical methods. The barriers that prevent translating this sensing strategy into clinics are outlined together with the potential solutions. We also share our vision on how multiplexed immunosensors will continue their evolution in the coming years.
准确、可靠且具有成本效益的免疫传感器对于疾病的早期诊断和监测具有重要的临床意义，而多重传感是下一代诊断的一种很有前途的策略。这种策略允许同时检测和定量多个生物标志物，具有显著提高的可重复性和可靠性，同时与单个测试相比，需要更小的样本量、更少的材料和更短的单个生物标志物的平均分析时间。在这个有意见的综述中，我们比较了用于开发多重免疫传感器的不同技术。我们综述了使用电学、电化学和光学方法的多重免疫传感器领域的最新进展。概述了将这种传感策略转化为临床应用的障碍，以及潜在的解决方案。我们还分享了我们对未来几年多重免疫传感器将如何继续发展的看法。
