Sulzer A J, Franco E L, Takafuji E, Benenson M, Walls K W, Greenup R L
Am J Trop Med Hyg. 1986 Mar;35(2):290-6. doi: 10.4269/ajtmh.1986.35.290.
Sera from 32 patients who became ill after jungle combat training were tested for antibodies to Toxoplasma gondii using the indirect immunofluorescence test. Swift rises of both IgG and IgM antibodies occurred within 2 weeks of infection. Reduction in IgM titers, due to competitive suppression by IgG antibody, occurred in most but not all cases. Suppression of IgM reaction by IgG antibody could be prevented by adsorption of serum with Staphylococcus aureus containing protein A. Antibody of the IgM class could be detected at greater than or equal 1:256 level in many sera at 6-month and 1-year intervals after exposure. In groups with exposures such as were experienced in this study, the presence of IgM antibody titers in single serum specimens cannot be used to indicate recent exposure. Both IgG and IgM antibody may rise together to high levels very rapidly after infection; IgM did not precede IgG antibody in our 32 subjects.
对32名在丛林作战训练后患病的患者的血清进行检测，采用间接免疫荧光试验检测弓形虫抗体。感染后2周内，IgG和IgM抗体迅速升高。在大多数但并非所有病例中，由于IgG抗体的竞争性抑制，IgM滴度降低。用含蛋白A的金黄色葡萄球菌吸附血清可防止IgG抗体对IgM反应的抑制。在接触后6个月和1年的许多血清中，可检测到IgM类抗体水平大于或等于1:256。在本研究中经历的此类接触组中，单次血清标本中IgM抗体滴度的存在不能用于表明近期接触。感染后，IgG和IgM抗体可能会一起迅速升高至高水平；在我们的32名受试者中，IgM并未先于IgG抗体出现。
