朱利弗洛林的抗皮肤真菌活性体外研究及其在沙门氏菌/微粒体试验系统中作为致癌物的筛选。

In vitro studies of antidermatophytic activity of juliflorine and its screening as carcinogen in Salmonella/microsome test system.

作者信息

Khursheed A K, Arshad H F, Viqaruddin A, Sabiha Q, Sheikh A R, Tahir S H

出版信息

Arzneimittelforschung. 1986;36(1):17-9.

Abstract

Juliflorine, an alkaloid from Prosopis juliflora, was tested for its antifungal activity against the freshly isolated cultures of dermatophytic fungi and its inhibitory effect was compared with that of griseofulvin. The results indicated that the minimum inhibitory concentration (MIC) of juliflorine against Trichophyton rubrum, Trichophyton violaceum, Trichophyton mentagrophytes, Trichophyton tonsurans, Trichophyton megninii, Trichophyton gallinae, Microsporum canis, Microsporum nanum, Microsporum ferrugineum and Epidermophyton floccosum was 1.5 micrograms/ml, whereas that of griseofulvin was 0.1-0.5 microgram/ml. MIC of juliflorine for Candida albicans was 0.05 mg/ml. The alkaloid was also subjected to screening for carcinogenicity in the Ames test (salmonella/microsome test system). The results indicated that the compound in the recommended concentrations did not exhibit positive mutagenic reaction, as compared to a strong positive reaction by ethyl methane sulfonate based on his- ----his+ revertants.

摘要

金合欢碱是一种从牧豆树中提取的生物碱，对新分离出的皮肤癣菌培养物进行了抗真菌活性测试，并将其抑制效果与灰黄霉素进行了比较。结果表明，金合欢碱对红色毛癣菌、紫色毛癣菌、须癣毛癣菌、断发毛癣菌、麦格尼毛癣菌、鸡禽毛癣菌、犬小孢子菌、纳米小孢子菌、铁锈色小孢子菌和絮状表皮癣菌的最低抑菌浓度（MIC）为1.5微克/毫升，而灰黄霉素的最低抑菌浓度为0.1 - 0.5微克/毫升。金合欢碱对白色念珠菌的最低抑菌浓度为0.05毫克/毫升。该生物碱还通过艾姆斯试验（沙门氏菌/微粒体试验系统）进行了致癌性筛查。结果表明，与基于组氨酸缺陷型向组氨酸野生型回复突变体的甲磺酸乙酯产生的强阳性反应相比，该化合物在推荐浓度下未表现出阳性诱变反应。