Faculty of Pharmacy, Université de Montréal, PO Box 6128, Succursale Centre-ville, Montréal, Québec H3C 3J7, Canada.
Department of Chemical Engineering, Polytechnique Montréal, Montreal H3C 3A7, Canada.
ACS Nano. 2022 Feb 22;16(2):1689-1707. doi: 10.1021/acsnano.1c05901. Epub 2022 Feb 9.
This Review aims to provide a systematic analysis of the literature regarding ongoing debates in protein corona research. Our goal is to portray the current understanding of two fundamental and debated characteristics of the protein corona, namely, the formation of mono- or multilayers of proteins and their binding (ir)reversibility. The statistical analysis we perform reveals that these characterisitics are strongly correlated to some physicochemical factors of the NP-protein system (particle size, bulk material, protein type), whereas the technique of investigation or the type of measurement ( or ) do not impact the results, unlike commonly assumed. Regarding the binding reversibility, the experimental design (either dilution or competition experiments) is also shown to be a key factor, probably due to nontrivial protein binding mechanisms, which could explain the paradoxical phenomena reported in the literature. Overall, we suggest that to truly predict and control the protein corona, future efforts should be directed toward the mechanistic aspects of protein adsorption.
这篇综述旨在对蛋白质冠研究中正在进行的争论的文献进行系统分析。我们的目标是描绘当前对蛋白质冠两个基本且有争议的特征的理解，即蛋白质的单分子或多分子层的形成及其结合的（不可）逆转性。我们进行的统计分析表明，这些特征与 NP-蛋白质系统的某些物理化学因素（颗粒大小、本体材料、蛋白质类型）强烈相关，而调查技术或测量类型（动态光散射或原子力显微镜）则不会影响结果，这与通常的假设不同。关于结合的可逆性，实验设计（稀释或竞争实验）也被证明是一个关键因素，这可能是由于蛋白质结合机制的复杂性，可以解释文献中报道的矛盾现象。总的来说，我们建议，为了真正预测和控制蛋白质冠，未来的研究应该针对蛋白质吸附的机制方面。
