Yang Qing, Chen Binlong, Robinson Kelsy, Belem Thiago, Lyu Wentao, Deng Zhuo, Ramanathan Ranjith, Zhang Guolong
Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK, USA.
College of Animal Science, Xichang University, Xichang, Sichuan, China.
J Anim Sci Biotechnol. 2022 Feb 10;13(1):3. doi: 10.1186/s40104-021-00663-2.
The emergence of antimicrobial resistance has necessitated the development of effective alternatives to antibiotics for livestock and poultry production. This study investigated a possible synergy between butyrate and forskolin (a natural labdane diterpene) in enhancing innate host defense, barrier function, disease resistance, growth performance, and meat quality of broilers.
The expressions of representative genes involved in host defense (AvBD9 and AvBD10), barrier function (MUC2, CLDN1, and TJP1), and inflammation (IL-1β) were measured in chicken HD11 macrophages in response to butyrate and forskolin in the presence or absence of bacterial lipopolysaccharides (LPS). Intestinal lesions and the Clostridium perfringens titers were also assessed in C. perfringens-challenged chickens fed butyrate and forskolin-containing Coleus forskohlii (CF) extract individually or in combination. Furthermore, growth performance and carcass characteristics were evaluated in broilers supplemented with butyrate and the CF extract for 42 d.
Butyrate and forskolin synergistically induced the expressions of AvBD9, AvBD10, and MUC2 in chicken HD11 cells (P < 0.05) and the synergy was maintained in the presence of LPS. Butyrate and forskolin also suppressed LPS-induced IL-1β gene expression in HD11 cells in a synergistic manner (P < 0.05). The two compounds significantly reduced the intestinal lesions of C. perfringens-challenged chickens when combined (P < 0.05), but not individually. Furthermore, butyrate in combination with forskolin-containing CF extract had no influence on weight gain, but significantly reduced feed intake (P < 0.05) with a strong tendency to improve feed efficiency (P = 0.07) in a 42-d feeding trial. Desirably, the butyrate/forskolin combination significantly decreased abdominal fat deposition (P = 0.01) with no impact on the carcass yield, breast meat color, drip loss, or pH of d-42 broilers.
Butyrate and forskolin has potential to be developed as novel antibiotic alternatives to improve disease resistance, feed efficiency, and carcass composition of broilers.
抗菌药物耐药性的出现使得有必要开发用于畜禽生产的有效抗生素替代品。本研究调查了丁酸盐和福斯可林（一种天然半日花烷二萜）在增强肉鸡先天宿主防御、屏障功能、抗病能力、生长性能和肉质方面可能存在的协同作用。
在存在或不存在细菌脂多糖（LPS）的情况下，检测丁酸盐和福斯可林作用于鸡HD11巨噬细胞时，参与宿主防御（AvBD9和AvBD10）、屏障功能（MUC2、CLDN1和TJP1）以及炎症（IL-1β）的代表性基因的表达。还评估了单独或联合饲喂含丁酸盐和福斯可林的毛喉鞘蕊花（CF）提取物的产气荚膜梭菌攻毒鸡的肠道病变和产气荚膜梭菌滴度。此外，对补充丁酸盐和CF提取物42天的肉鸡的生长性能和胴体特性进行了评估。
丁酸盐和福斯可林协同诱导鸡HD11细胞中AvBD9、AvBD10和MUC2的表达（P < 0.05），并且在存在LPS的情况下这种协同作用得以维持。丁酸盐和福斯可林还以协同方式抑制HD11细胞中LPS诱导的IL-1β基因表达（P < 0.05）。这两种化合物联合使用时能显著降低产气荚膜梭菌攻毒鸡的肠道病变（P < 0.05），但单独使用时无此效果。此外，在为期42天的饲养试验中丁酸盐与含福斯可林的CF提取物联合使用对体重增加没有影响，但显著降低了采食量（P < 0.05），并且有提高饲料效率的强烈趋势（P = 0.07）。理想情况下丁酸盐/福斯可林组合显著降低了腹部脂肪沉积（P = 0.01），对42日龄肉鸡的胴体产量、胸肉颜色、滴水损失或pH值没有影响。
丁酸盐和福斯可林有潜力被开发为新型抗生素替代品，以提高肉鸡的抗病能力、饲料效率和胴体组成。
