Yang Qing, Whitmore Melanie A, Robinson Kelsy, Lyu Wentao, Zhang Guolong
Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
Poultry Production and Product Safety Research Unit, USDA-Agricultural Research Service, Fayetteville, AR 72701, USA.
Antibiotics (Basel). 2021 Sep 27;10(10):1175. doi: 10.3390/antibiotics10101175.
The rising concern of antimicrobial resistance highlights a need for effective alternatives to antibiotics for livestock production. Butyrate, forskolin, and lactose are three natural products known to induce the synthesis of host defense peptides (HDP), which are a critical component of innate immunity. In this study, the synergy among butyrate, forskolin, and lactose in enhancing innate host defense, barrier function, and resistance to necrotic enteritis and coccidiosis was investigated. Our results indicated that the three compounds synergistically augmented the expressions of multiple HDP and barrier function genes in chicken HD11 macrophages. The compounds also showed an obvious synergy in promoting HDP gene expressions in chicken jejunal explants. Dietary supplementation of a combination of 1 g/kg sodium butyrate, 10 mg/kg forskolin-containing plant extract, and 10 g/kg lactose dramatically improved the survival of chickens from 39% to 94% ( < 0.001) in a co-infection model of necrotic enteritis. Furthermore, the three compounds largely reversed growth suppression, significantly alleviated intestinal lesions, and reduced colonization of or in chickens with necrotic enteritis and coccidiosis ( < 0.01). Collectively, dietary supplementation of butyrate, forskolin, and lactose is a promising antibiotic alternative approach to disease control and prevention for poultry and possibly other livestock species.
对抗菌素耐药性日益增长的关注凸显了在畜牧生产中需要有效的抗生素替代品。丁酸盐、福斯可林和乳糖是三种已知可诱导宿主防御肽（HDP）合成的天然产物，而宿主防御肽是先天免疫的关键组成部分。在本研究中，研究了丁酸盐、福斯可林和乳糖在增强宿主先天防御、屏障功能以及对坏死性肠炎和球虫病的抵抗力方面的协同作用。我们的结果表明，这三种化合物协同增强了鸡HD11巨噬细胞中多种宿主防御肽和屏障功能基因的表达。这些化合物在促进鸡空肠外植体中宿主防御肽基因表达方面也表现出明显的协同作用。在坏死性肠炎共感染模型中，日粮中添加1 g/kg丁酸钠、10 mg/kg含福斯可林的植物提取物和10 g/kg乳糖的组合可将鸡的存活率从39%显著提高到94%（<0.001）。此外，这三种化合物在很大程度上逆转了生长抑制，显著减轻了肠道病变，并减少了坏死性肠炎和球虫病鸡体内产气荚膜梭菌或柔嫩艾美耳球虫的定植（<0.01）。总体而言，日粮中添加丁酸盐、福斯可林和乳糖是一种很有前景的抗生素替代方法，可用于家禽以及可能其他家畜物种的疾病控制和预防。
