Sun Shun Yu, Yang Wei Yi, Tan Zhuo, Zhang Xing Yu, Shen Yi Lin, Guo Qi Wei, Su Guo Ming, Chen Xu, Lin Jia, Fang Ding Zhi
Department of Biochemistry and Molecular Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, 610041, People's Republic of China.
Department of Preventive Medicine, West China School of Public health, Sichuan University, Chengdu, 610041, People's Republic of China.
Diabetes Metab Syndr Obes. 2022 Feb 3;15:331-343. doi: 10.2147/DMSO.S348800. eCollection 2022.
To investigate serum levels of free fatty acids (FFAs) and their associations with routine serum lipids in diet-induced obese mice, which have been scantily reported before.
Male C57BL/6 J mice were fed high-fat diets for 12 weeks to induce obesity. Levels of serum FFAs were measured by ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.
Obese mice had higher serum levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), but lower triglycerides (TG) than control mice. A total of 30 FFAs were found, and 3 saturated fatty acids (SFAs), all 8 monounsaturated fatty acids (MUFAs) and 7 polyunsaturated fatty acids (PUFAs) decreased in obese mice, but one SFA (C4:0) increased. Differences in the relative levels of individual FFAs to total FFAs, SFAs, MUFAs or PUFAs between obese and control mice were different from each other and from those evaluated by concrete levels except C4:0, C16:1, C19:1 and C18:4. Only the concrete levels of C4:0, C22:3 and C18:4 were associated with routine serum lipids, including C22:3 negatively with TG in control mice, and C4:0 and C18:4 positively with LDL-C in obese mice, although the relative levels of C4:0 to total MUFAs negatively with TC, and C23:3 to total SFAs or MUFAs negatively with TG in control mice. Different relative levels of the remaining FFAs were differently associated with different routine serum lipids in obese and/or control mice.
Obesity may influence serum FFAs profiles. The relationship of individual FFAs and their relative levels to other FFAs with routine serum lipids in obese and control mice suggests that individual FFAs may interact with others and obesity on levels of routine serum lipids. Once confirmed, the interactions may be novel perspectives when fatty acids are used to improve hyperlipidemia in the subjects with obesity.
研究饮食诱导肥胖小鼠的血清游离脂肪酸（FFA）水平及其与常规血脂的关系，此前相关报道较少。
雄性C57BL/6 J小鼠喂食高脂饮食12周以诱导肥胖。采用超高效液相色谱 - 电喷雾电离 - 串联质谱法测定血清FFA水平。
肥胖小鼠的血清总胆固醇（TC）、高密度脂蛋白胆固醇（HDL - C）和低密度脂蛋白胆固醇（LDL - C）水平高于对照小鼠，但甘油三酯（TG）水平低于对照小鼠。共检测到30种FFA，肥胖小鼠中3种饱和脂肪酸（SFA）、所有8种单不饱和脂肪酸（MUFA）和7种多不饱和脂肪酸（PUFA）水平降低，但一种SFA（C4:0）升高。肥胖小鼠与对照小鼠之间，个体FFA相对于总FFA、SFA、MUFA或PUFA的相对水平差异，与除C4:0、C16:1、C19:1和C18:4外通过具体水平评估的差异不同。仅C4:0、C22:3和C18:4的具体水平与常规血脂相关，包括对照小鼠中C22:3与TG呈负相关，肥胖小鼠中C4:0和C18:4与LDL - C呈正相关，尽管对照小鼠中C4:0相对于总MUFA与TC呈负相关，C23:3相对于总SFA或MUFA与TG呈负相关。其余FFA的不同相对水平在肥胖和/或对照小鼠中与不同的常规血脂有不同的相关性。
肥胖可能影响血清FFA谱。肥胖和对照小鼠中个体FFA及其相对水平与其他FFA和常规血脂的关系表明，个体FFA可能在常规血脂水平上与其他FFA及肥胖相互作用。一旦得到证实，这些相互作用可能为使用脂肪酸改善肥胖受试者的高脂血症提供新的视角。
