过氧一硫酸氢钾复合盐从还原胺化标记聚糖中再生游离还原糖
Regeneration of Free Reducing Glycans from Reductive Amination-Tagged Glycans by Oxone.
机构信息
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, United States.
Department of Chemistry, Georgia State University, Atlanta, Georgia 30302, United States.
出版信息
J Org Chem. 2022 Mar 4;87(5):3736-3740. doi: 10.1021/acs.joc.1c02709. Epub 2022 Feb 10.
Abstract
Glycans are usually fluorescently tagged by reductive amination for analytic tools. However, free reducing glycan regeneration is sometimes important and necessary for further structural or functional studies. Here, we introduce a new method for efficiently removing fluorescent tags from glycoconjugates by a simple treatment with Oxone. This method is proven to be fast and general after being tested on a series of common saccharides and widely used tags. We successfully achieved -glycopeptide synthesis by using the regenerated glycans.
摘要
糖通常通过还原胺化反应进行荧光标记，以便于分析工具使用。然而，对于进一步的结构或功能研究，有时需要对游离还原糖进行再生。在此，我们介绍了一种通过使用 Oxone 简单处理从糖缀合物中有效去除荧光标记的新方法。该方法在一系列常见的糖和广泛使用的标记物上进行测试后，被证明快速且通用。我们成功地使用再生糖实现了 -糖肽的合成。
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