Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, People's Republic of China.
The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, People's Republic of China.
Anal Chem. 2022 Feb 22;94(7):3418-3426. doi: 10.1021/acs.analchem.2c00066. Epub 2022 Feb 11.
Point-of-care testing (POCT) technology has made major breakthroughs in community medicine and physician office situations, in tandem with the more ubiquitous and intensive usage of highly integrated quick detection equipment for illness diagnosis, personal care, and mobile healthcare. Although the photoelectrochemical (PEC)-based POCT platform offers the benefits of cheap cost and good user engagement, its commercialization is still limited by the photodetection components' downsizing and mobility, among other factors. In this work, a novel highly integrated PEC biosensor aided by piezophototronics to enhance the efficiency of PEC testing was reported for flexible detection of cancer-associated antigens in biological fluids (prostate-specific antigen, PSA, used as an example). Multiple signal enhancement strategies, including a magnetic bead-linked enzyme-linked immune system catalyzing the production of ascorbic acid from the substrate and a piezoelectric-assisted enhancement strategy, were used for sensitive detection of the analyte to be tested in human body fluids. Unlike the electron transfer mechanism in heterojunctions, piezoelectric semiconductors promote the transfer of electrons and holes by generating piezoelectric potentials in the ultrasonic field, thus contributing to the performance of the PEC testbed. Under optimized conditions, the test platform achieves good correspondence for PSA at 0.02-40 ng mL. Impressively, the test devices are comparable to or even superior to gold standard ELISA kits in terms of cost approval and batch testing. This research demonstrates the potential of piezoelectric semiconductors for POC applications in revolutionary PECs and offers innovative thoughts for the development of new PEC bioanalytical components.
即时检测（POCT）技术在社区医学和医师诊室环境中取得了重大突破，这与更广泛和更深入使用高度集成的快速检测设备进行疾病诊断、个人护理和移动医疗保健是分不开的。虽然基于光电化学（PEC）的 POCT 平台具有成本低、用户参与度高的优点，但由于光探测组件的小型化和移动性等因素，其商业化仍然受到限制。在这项工作中，报道了一种新型的高度集成的 PEC 生物传感器，该传感器利用压电器件来增强 PEC 测试的效率，用于在生物流体中（以前列腺特异性抗原 PSA 为例）灵活检测癌症相关抗原。采用了多种信号增强策略，包括磁珠连接的酶联免疫系统，从基质中催化产生抗坏血酸，以及压电辅助增强策略，用于灵敏检测人体生物流体中的分析物。与异质结中的电子转移机制不同，压电半导体通过在超声场中产生压电电势来促进电子和空穴的转移，从而有助于提高 PEC 测试平台的性能。在优化条件下，该测试平台对 PSA 的检测范围为 0.02-40ng/mL，具有良好的相关性。令人印象深刻的是，测试设备在成本审批和批量测试方面与金标准 ELISA 试剂盒相当，甚至更具优势。这项研究展示了压电半导体在革命性 PEC 中的 POCT 应用中的潜力，并为开发新型 PEC 生物分析组件提供了创新思路。
