Tan Qinqin, Wang Yue, Liu Ying, Tao Zhongfa, Yu Chun, Huang Yan, Yang Xinggui, Ying Xia, Hu Yong, Li Shijun
Laboratory Center, Center for Disease Control and Prevention of Guizhou Provincial, Guiyang, Guizhou, China.
The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, University of Guizhou Medical, Guiyang, Guizhou, China.
Front Microbiol. 2023 Jun 23;14:1188469. doi: 10.3389/fmicb.2023.1188469. eCollection 2023.
Brucellosis was made statutorily notifiable in 1955, in China, while in Guizhou Province, the pathogen of human brucellosis was isolated for the first time in 2011. However, currently, the brucellosis epidemic is becoming more and more severe in Guizhou Province. The type distribution and genetic characteristics of in Guizhou Province, as well as its evolutionary relationship with domestic and foreign strains, are still unclear.
MLST, MLVA, and typing techniques were used for the molecular epidemiological study of the 83 isolates in Guizhou province.
Among the 83 strains, MLST identified three ST genotypes, of which ST39 is a newly reported type in China. MLVA-16 generated 49 genotypes, and MLVA-11 generated 5 known genotypes and 2 unreported genotypes. Six genotypes were identified by technology.
MLVA has a high resolution, but differences at the Bruce 04 and 16 loci cannot exclude associations between epidemics, and combining MLST and typing methods for epidemiologic tracing can avoid erroneous judgments. Moreover, through the combined analysis of the three typing techniques, the possible origin of the new can be reasonably inferred, which is also conducive to promoting the subsequent research of the novel .
布鲁氏菌病于1955年在中国被列为法定报告传染病，而在贵州省，2011年首次分离出人类布鲁氏菌病病原体。然而，目前贵州省布鲁氏菌病疫情日益严峻。贵州省布鲁氏菌的型别分布、遗传特征及其与国内外菌株的进化关系仍不清楚。
采用多位点序列分型（MLST）、多位点可变数目串联重复序列分析（MLVA）和脉冲场凝胶电泳（PFGE）分型技术对贵州省83株布鲁氏菌进行分子流行病学研究。
83株布鲁氏菌中，MLST鉴定出3种序列型基因型，其中ST39是中国新报道的型别。MLVA-16产生49种基因型，MLVA-11产生5种已知基因型和2种未报道的基因型。PFGE技术鉴定出6种基因型。
MLVA分辨率高，但布鲁氏菌04和16位点的差异不能排除疫情之间的关联，联合MLST和PFGE分型方法进行流行病学溯源可避免错误判断。此外，通过三种分型技术的联合分析，可合理推断新型布鲁氏菌的可能来源，也有利于推动新型布鲁氏菌后续研究。
