Wibisono Freshindy Marissa, Faridah Hayyun Durrotul, Wibisono Freshinta Jellia, Tyasningsih Wiwiek, Effendi Mustofa Helmi, Witaningrum Adiana Mutamsari, Ugbo Emmanuel Nnabuike
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.
Vet World. 2021 Dec;14(12):3126-3131. doi: 10.14202/vetworld.2021.3126-3131. Epub 2021 Dec 19.
The increasing number of multidrug-resistant (MDR) species on poultry farms in Indonesia has caused concern regarding human health. This study was conducted to determine the presence of the virulence gene A in MDR species isolated from the cloacal swab of broiler chickens in Blitar district, East Java Province, Indonesia.
Cloacal swab samples were collected by purposive sampling from 15 farms in four districts. Isolation and identification of bacteria were performed using standard microbiological techniques. Confirmation of MDR isolates was done using five different classes of antibiotics, including the beta-lactam, aminoglycoside, fluoroquinolone, phenicol, and monobactam groups. An antibiotic susceptibility test was conducted using the Kirby-Bauer disk diffusion method, and a polymerase chain reaction method was used to screen for the presence of A.
It was observed that 32.26% (50/155) of the samples were positive for species. Of these 50 isolates, 7 (14%) were identified as MDR strains. An important finding was the detection of A in all the seven MDR strains (100%) isolated from the cloacal swab of broiler chickens in Blitar district, East Java Province.
Veterinarians have an extremely important role in monitoring the use of antibiotics in farm animals to mitigate the rapid spread of MDR organisms in our environment, which can otherwise cause serious economic losses and also public health issues.
印度尼西亚家禽养殖场中多重耐药（MDR）菌株数量的增加引发了对人类健康的担忧。本研究旨在确定从印度尼西亚东爪哇省勿里达区肉鸡泄殖腔拭子中分离出的MDR菌株中是否存在毒力基因A。
通过目的抽样从四个区的15个养殖场采集泄殖腔拭子样本。使用标准微生物技术进行细菌的分离和鉴定。使用包括β-内酰胺类、氨基糖苷类、氟喹诺酮类、氯霉素类和单环β-内酰胺类在内的五种不同类别的抗生素对MDR分离株进行确认。采用 Kirby-Bauer 纸片扩散法进行抗生素敏感性试验，并使用聚合酶链反应法筛选A的存在情况。
观察到32.26%（50/155）的样本对[具体细菌名称]呈阳性。在这50株[具体细菌名称]分离株中，有7株（14%）被鉴定为MDR菌株。一个重要发现是，从印度尼西亚东爪哇省勿里达区肉鸡泄殖腔拭子中分离出的所有七株MDR[具体细菌名称]菌株（100%）均检测到A。
兽医在监测农场动物抗生素使用方面发挥着极其重要的作用，以减轻MDR生物体在我们环境中的快速传播，否则可能会造成严重的经济损失以及公共卫生问题。
