Lv Bo, Peng Yuan-de, Fu Wen-Hui, Li Wen-Jing, Chen Li-Jun, Wang Zhi
Key Laboratory of Protein Chemistry and Developmental Biology of Fish of Ministry of Education, Hunan Normal University, Changsha 410081, China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, Hunan, China.
Evid Based Complement Alternat Med. 2022 Feb 2;2022:5200608. doi: 10.1155/2022/5200608. eCollection 2022.
Dysfunctional uterine bleeding, accompanied by endometrial hyperplasia (EH), is a common gynecological disease that seriously affects female physical and mental health. Some drugs have been prompted to cure the disease, but most medications have certain side effects and limitations. In the present study, we demonstrated an unexploited Chinese traditional medicine, a combination of , , and (SCS), which could be used for the treatment of EH and associated complications in rats. We identified the active components from the three Chinese herbs via thin-layer chromatography and high-performance liquid chromatography methods. In addition, serum biochemical indexes and histologic section results found that acute high-dose SCS exerted no adverse impacts on the rats. We then showed that SCS shortened coagulation time (=0.018) and degree of swelling (=0.021) on rats at 30 min compared to blank control. Further studies proved that recovered endometrial thickness was associated with the modulation of four hormones (follicle-stimulating hormone, luteinizing hormone, estrogen, and progesterone). Specifically, follicle-stimulating hormone and progesterone contents increased gradually with time, and estrogen was decreased, whereas luteinizing hormone content was returned to normal after a short-term elevation ( < 0.05). Besides, SCS increased uterine endometrium's mRNA expression levels of matrix metalloproteinase-1 ( < 0.001) and tissue inhibitor of matrix metalloproteinase-1 ( < 0.001), promoting the repair of proliferating endometrium in the rats. Collectively, our study indicates that SCS harbors a profoundly curative effect on the treatment of EH and relative complications and uncovers the mechanism at molecular and gene expression levels.
功能失调性子宫出血伴子宫内膜增生（EH）是一种常见的妇科疾病，严重影响女性身心健康。一些药物已被用于治疗该疾病，但大多数药物都有一定的副作用和局限性。在本研究中，我们展示了一种未被开发的中药，即[三种中药名称]的组合（SCS），它可用于治疗大鼠的EH及其相关并发症。我们通过薄层色谱法和高效液相色谱法从这三种中药中鉴定出了活性成分。此外，血清生化指标和组织切片结果表明，急性高剂量SCS对大鼠没有不良影响。然后我们发现，与空白对照组相比，SCS在30分钟时缩短了大鼠的凝血时间（=0.018）和肿胀程度（=0.021）。进一步的研究证明，子宫内膜厚度的恢复与四种激素（促卵泡激素、促黄体生成素、雌激素和孕酮）的调节有关。具体而言，促卵泡激素和孕酮含量随时间逐渐增加，雌激素减少，而促黄体生成素含量在短期升高后恢复正常（<0.05）。此外，SCS增加了子宫子宫内膜基质金属蛋白酶-1（<0.001）和基质金属蛋白酶组织抑制剂-1（<0.001）的mRNA表达水平，促进了大鼠增生期子宫内膜的修复。总的来说，我们的研究表明SCS对EH及其相关并发症具有显著的治疗效果，并在分子和基因表达水平上揭示了其作用机制。
