Wu Chung-Hsin, Wang Sheue-Er, Hsu Chih-Hsiang, Hsu Yu-Tsen, Lu Chen-Wen, Chuang Wu-Chang, Lee Ming-Chung
School of Life Science, National Taiwan Normal University, Taipei City, Taiwan.
Pathological Department, Saint Paul's Hospital, Taoyuan City, Taiwan.
Evid Based Complement Alternat Med. 2019 Jul 7;2019:5346518. doi: 10.1155/2019/5346518. eCollection 2019.
In Taiwan, the herbal formula B401 is considered as a health supplement for middle-aged women that can alleviate sweating, anxiety, and sleep disorders. However, the relevant mechanisms are still unclear. In this study, we evaluated the beneficial effects of the herbal formula B401 therapy in the reproductive regulation of ovariectomised mice. Female ICR mice were randomised into four groups: wild-type (WT) mice with sham treatment, wild-type mice treated with the herbal formula B401, bilateral ovariectomised (OVX) mice with sham treatment, and bilateral ovariectomised mice treated with the herbal formula B401. Mice were orally given the herbal formula B401 at a dose of 30 mg/kg bw/day for 2 weeks. At the end of oral treatment with sham or the herbal formula B401, levels of reactive oxygen species (ROS), calcium, phosphorus, and estradiol-17 in the blood; uterine weight and endometrial thickness; and expressions of estrogen receptor (ER), estrogen receptor (ER), progesterone receptor (PR), vascular endothelial growth factor (VEGF), and superoxide dismutase 2 (SOD2) in the uterine tissue were examined and then compared among the four groups of mice. We found that OVX mice decreased levels of calcium, phosphorus, and estradiol-17 in the blood, decreased uterine weight and endometrial thickness, and decreased expressions of ER, ER, PR, and SOD2 in the uterine tissue but increased blood ROS levels compared with those of WT mice. In addition, OVX mice with the herbal formula B401 therapy can increase levels of calcium, phosphorus, and estradiol-17 in the blood, increase uterine weight and endometrial thickness, and increase expressions of ER, ER, PR, VEGF, and SOD2 in the uterine tissue but decrease blood ROS levels. Our results may provide reasonable explanation for the reproductive regulation of the herbal formula B401 therapy.
在台湾，中药配方B401被视为一种可缓解出汗、焦虑和睡眠障碍的中年女性健康补充剂。然而，相关机制仍不清楚。在本研究中，我们评估了中药配方B401疗法对去卵巢小鼠生殖调节的有益作用。将雌性ICR小鼠随机分为四组：假手术处理的野生型（WT）小鼠、用中药配方B401处理的野生型小鼠、假手术处理的双侧去卵巢（OVX）小鼠和用中药配方B401处理的双侧去卵巢小鼠。小鼠按30 mg/kg体重/天的剂量口服中药配方B401，持续2周。在假手术或中药配方B401口服治疗结束时，检测血液中活性氧（ROS）、钙、磷和雌二醇-17的水平；子宫重量和子宫内膜厚度；以及子宫组织中雌激素受体（ER）、雌激素受体（ER）、孕激素受体（PR）、血管内皮生长因子（VEGF）和超氧化物歧化酶2（SOD2）的表达，然后在四组小鼠之间进行比较。我们发现，与WT小鼠相比，OVX小鼠血液中钙、磷和雌二醇-17的水平降低，子宫重量和子宫内膜厚度降低，子宫组织中ER、ER、PR和SOD2的表达降低，但血液ROS水平升高。此外，接受中药配方B401治疗的OVX小鼠可提高血液中钙、磷和雌二醇-17的水平，增加子宫重量和子宫内膜厚度，增加子宫组织中ER、ER、PR、VEGF和SOD2的表达，但降低血液ROS水平。我们的结果可能为中药配方B401疗法的生殖调节提供合理的解释。
