Gao Zhaolin, Tan Jia, Wang Sha, Yu Haiyang, Zhou Ziyu, Zhang Yun, Zhou Mushi, Xia Xiaobo, Yao Fei, Huang Jufang
Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, China.
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China.
Front Med (Lausanne). 2022 Jan 27;8:774624. doi: 10.3389/fmed.2021.774624. eCollection 2021.
The pathogenesis and etiology of various ocular tumors remain largely unclear, limiting the development of diagnostic and treatment approaches for such tumors. Tissue samples from patients are also valuable resource to elucidate mechanisms underlying tumorigenesis. Here we present the early phase setup of an ocular tumor biobank at Xiangya Hospital. Blood and tissue samples along with associated clinical data were obtained from patients who underwent surgery in the Department of Ophthalmology of Xiangya Hospital from December 1, 2018 to January 31, 2020. Standardized operating protocols were developed for the collection, transportation, processing and preservation of ocular tumor samples. A total of 92 clinical cases suffered from 21 types of eye tumors and several undiagnosed eye diseases were covered. A total of 846 samples were preserved in the ocular tumor biobank, including 356 blood samples (42.1%), 324 plasma samples (38.3%), and 166 tissue samples (19.6%). Using the clinical data, we analyzed the prevalence of malignant ocular tumors in association with variables of age, gender, tumors' location and size, and presenting complaints of lump and proptosis. The factors predictive of malignant ocular tumors, included gender (B = 1.599; = 0.025) and the symptom of proptosis (B = -2.534; = 0.001). Overall, the setup of clinically-based ophthalmologic biobank could support pathological and translational research into ocular tumors.
各种眼部肿瘤的发病机制和病因在很大程度上仍不明确，这限制了此类肿瘤诊断和治疗方法的发展。患者的组织样本也是阐明肿瘤发生机制的宝贵资源。在此，我们展示了湘雅医院眼部肿瘤生物样本库的早期建设情况。从2018年12月1日至2020年1月31日在湘雅医院眼科接受手术的患者中获取了血液和组织样本以及相关临床数据。针对眼部肿瘤样本的采集、运输、处理和保存制定了标准化操作方案。共涵盖了92例患有21种眼部肿瘤类型以及几种未确诊眼病的临床病例。眼部肿瘤生物样本库共保存了846份样本，包括356份血液样本（42.1%）、324份血浆样本（38.3%）和166份组织样本（19.6%）。利用临床数据，我们分析了恶性眼部肿瘤的患病率与年龄、性别、肿瘤位置和大小以及肿块和眼球突出等症状表现变量之间的关系。预测恶性眼部肿瘤的因素包括性别（B = 1.599；P = 0.025）和眼球突出症状（B = -2.534；P = 0.001）。总体而言，基于临床的眼科生物样本库的建设可为眼部肿瘤病理和转化研究提供支持。
