Institute for Basic Sciences, Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.
Institute for Basic Sciences, Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Biomed Res Int. 2022 Feb 4;2022:6989963. doi: 10.1155/2022/6989963. eCollection 2022.
This study is aimed at evaluating the effects of (SS) seed extract on cholestatic liver injury induced by bile duct ligation (BDL) in rats. Total polyphenols and flavonoids in SS seed extract were determined using a colorimetric assay, and their components were quantified using HPLC. Rats in four groups underwent BDL at the common bile duct and were treated for 21 days with either oral distilled water as vehicle, vitamin C, 100 mg/kg SS seed extract, or 200 mg/kg SS seed extract. Rats in the fifth group underwent abdominal incision without BDL and were treated with distilled water, and rats in the sixth group were healthy and received nothing. Finally, rats were sacrificed, blood samples were analyzed through biochemical methods, liver tissues were histologically assessed, and the expression of the TGF-1, iNOS, caspase-3, and -SMA genes in the liver was assessed through real-time PCR. BDL significantly increased, and SS seed extract significantly decreased the serum levels of bilirubin and liver function enzymes. Moreover, SS seed extract suppressed the expression of the TGF-1, iNOS, caspase-3, and -SMA genes, reduced the levels of nitric oxide, malondialdehyde, and protein carbonyl, and increased the levels of glutathione, total antioxidant capacity, and SOD and catalase enzyme activity in the serum and liver. Extract at a dose of 100 mg/kg had significant positive effects on liver morphology and parenchyma structure in a dose-dependent manner.
本研究旨在评估（SS）种子提取物对胆管结扎（BDL）诱导的大鼠胆汁淤积性肝损伤的影响。采用比色法测定 SS 种子提取物中的总多酚和类黄酮含量，并采用 HPLC 定量其成分。四组大鼠在胆总管处行 BDL，并接受以下 21 天的治疗：口服蒸馏水作为载体、维生素 C、100mg/kg SS 种子提取物或 200mg/kg SS 种子提取物。第五组大鼠行腹部切口但不进行 BDL，并接受蒸馏水治疗，第六组大鼠为健康对照且不接受任何处理。最后，处死大鼠，通过生化方法分析血样，对肝组织进行组织学评估，并通过实时 PCR 评估 TGF-1、iNOS、caspase-3 和 -SMA 基因在肝脏中的表达。BDL 显著增加，而 SS 种子提取物显著降低了血清胆红素和肝功能酶水平。此外，SS 种子提取物抑制了 TGF-1、iNOS、caspase-3 和 -SMA 基因的表达，降低了血清和肝脏中一氧化氮、丙二醛和蛋白羰基的水平，增加了谷胱甘肽、总抗氧化能力和 SOD 及过氧化氢酶的活性。在剂量依赖性方式下，100mg/kg 剂量的提取物对肝形态和实质结构具有显著的积极影响。
