特定序列寡核苷酸的酶促合成：酵母异-1-细胞色素c基因片段的合成
Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.
作者信息
Gillam S, Rottman F, Jahnke P, Smith M
出版信息
Proc Natl Acad Sci U S A. 1977 Jan;74(1):96-100. doi: 10.1073/pnas.74.1.96.
Abstract
The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.
摘要
构成酵母同工-1-细胞色素c基因片段的脱氧核糖寡核苷酸d（pT-T-A-G-C-A-G-A-A-C-C-G-G）已通过化学方法和主要是酶促方法相结合的方式合成。起始引物d（pT-T-A-G1）通过磷酸二酯法化学合成，并通过多核苷酸磷酸化酶催化的反应逐步延伸。
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Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.特定序列寡核苷酸的酶促合成：酵母异-1-细胞色素c基因片段的合成
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