School of Life Sciences, Shandong Province Key Laboratory of Applied Mycology, Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, P. R. China.
Chem Biodivers. 2022 Apr;19(4):e202100832. doi: 10.1002/cbdv.202100832. Epub 2022 Feb 28.
A new isovaleryl-monoterpene, pleurotusin A (1), and a new cyclopentenone derivative, pleurotusin B (7), together with five related known terpenoids (2-6), were isolated from the coculture broth of two edible fungi, Pleurotus ostreatus SY10 and Pleurotus eryngii SY302. The absolute configurations of 1 and 7 were elucidated by comprehensively using the density functional theory calculation of NMR and ECD data, DP4+ probability analysis, Mo (OAc) -based CD experiment and optical rotation. The antimicrobial activities of these compounds except for the unstable compound 7, were evaluated against two types of human-pathogenic fungi, Candida albicans and Cryptococcus neoformans, and three types of human-pathogenic bacteria, Staphylococcus aureus, Escherichia coli, and Shigella sp. Compound 1 displayed moderate activity against S. aureus with an MIC value of 90.3 μM. In addition, the antioxidant activities of high-yielding 2-6 were tested using DPPH, and compound 4 showed moderate activity with an EC value of 573 μM.
一种新的异戊酰-单萜类化合物，平菇素 A（1）和一种新的环戊烯酮衍生物，平菇素 B（7），以及五种相关的已知萜类化合物（2-6），从两种可食用真菌，平菇 SY10 和杏鲍菇 SY302 的共培养肉汤中分离得到。通过综合使用 NMR 和 ECD 数据的密度泛函理论计算、DP4+ 概率分析、Mo（OAc）基 CD 实验和旋光，阐明了 1 和 7 的绝对构型。除不稳定化合物 7 外，这些化合物对两种人类致病真菌，白色念珠菌和新生隐球菌，以及三种人类致病细菌，金黄色葡萄球菌、大肠杆菌和志贺氏菌进行了抗菌活性评价。化合物 1 对金黄色葡萄球菌显示出中等活性，MIC 值为 90.3 μM。此外，使用 DPPH 测试了高产的 2-6 的抗氧化活性，化合物 4 表现出中等活性，EC 值为 573 μM。
