Yuan Yanping, Chen Lei, Shi Ziyuan, Chen Jimin
Faculty of Materials and Manufacturing, Institute of Laser Engineering, Beijing University of Technology, Beijing 100124, China.
Key Laboratory of Trans-Scale Laser Manufacturing Technology, Beijing University of Technology, Ministry of Education, Beijing 100124, China.
Nanomaterials (Basel). 2022 Jan 25;12(3):391. doi: 10.3390/nano12030391.
The fabrication of high-precision scaffolds with excellent biocompatibility for tissue engineering has become a research hotspot. Two-photon polymerization (TPP) can break the optical diffraction limit and is used to fabricate high-resolution three-dimensional (3D) microstructures. In this study, the biological properties, and machinability of photosensitive gelatin methacrylate (GelMA) hydrogel solutions are investigated, and the biocompatibility of 3D scaffolds using a photosensitive GelMA hydrogel solution fabricated by TPP is also evaluated. The biological properties of photosensitive GelMA hydrogel solutions are evaluated by analyzing their cytotoxicity, swelling ratio, and degradation ratio. The experimental results indicate that: (1) photosensitive GelMA hydrogel solutions with remarkable biological properties and processability are suitable for cell attachment. (2) a micro/nano 3D printed scaffold with good biocompatibility is fabricated using a laser scanning speed of 150 μm/s, laser power of 7.8 mW, layer distance of 150 nm and a photosensitive GelMA hydrogel solution with a concentration of 12% (/). Micro/nano additive manufacturing will have broad application prospects in the tissue engineering field.
制造具有优异生物相容性的高精度组织工程支架已成为研究热点。双光子聚合（TPP）可以突破光学衍射极限，用于制造高分辨率三维（3D）微结构。在本研究中，研究了光敏甲基丙烯酸明胶（GelMA）水凝胶溶液的生物学性质和可加工性，并评估了使用TPP制备的光敏GelMA水凝胶溶液制成的3D支架的生物相容性。通过分析其细胞毒性、溶胀率和降解率来评估光敏GelMA水凝胶溶液的生物学性质。实验结果表明：（1）具有显著生物学性质和可加工性的光敏GelMA水凝胶溶液适合细胞附着。（2）使用150μm/s的激光扫描速度、7.8mW的激光功率、150nm的层间距和浓度为12%（/）的光敏GelMA水凝胶溶液制造出具有良好生物相容性的微/纳米3D打印支架。微/纳米增材制造在组织工程领域将具有广阔的应用前景。
