Kocaaga Banu, Kurkcuoglu Ozge, Tatlier Melkon, Dinler-Doganay Gizem, Batirel Saime, Güner Fatma Seniha
Department of Chemical Engineering, Istanbul Technical University, Maslak, Istanbul 34469, Turkey.
Department of Molecular Biology and Genetics, Istanbul Technical University, Maslak, Istanbul 34469, Turkey.
Polymers (Basel). 2022 Jan 24;14(3):460. doi: 10.3390/polym14030460.
Hypoalbuminemia can lead to poor and delayed wound healing, while it is also associated with acute myocardial infarction, heart failure, malignancies, and COVID-19. In elective surgery, patients with low albumin have high risks of postoperative wound complications. Here, we propose a novel cost-effective wound dressing material based on low-methoxy pectin and NaA-zeolite particles with controlled albumin release properties. We focused on both albumin adsorption and release phenomena for wounds with excess exudate. Firstly, we investigated albumin dynamics and calculated electrostatic surfaces at experimental pH values in water by using molecular dynamics methods. Then, we studied in detail pectin-zeolite hydrogels with both adsorption and diffusion into membrane methods using different pH values and albumin concentrations. To understand if uploaded albumin molecules preserved their secondary conformation in different formulations, we monitored the effect of pH and albumin concentration on the conformational changes in albumin after it was released from the hydrogels by using CD-UV spectroscopy analyses. Our results indicate that at pH 6.4, BSA-containing films preserved the protein's folded structure while the protein was being released to the external buffer solutions. In vitro wound healing assay indicated that albumin-loaded hydrogels showed no toxic effects on the fibroblast cells.
低白蛋白血症可导致伤口愈合不良和延迟，同时还与急性心肌梗死、心力衰竭、恶性肿瘤和新冠肺炎有关。在择期手术中，白蛋白水平低的患者术后伤口并发症风险较高。在此，我们提出了一种基于低甲氧基果胶和具有可控白蛋白释放特性的NaA沸石颗粒的新型经济高效的伤口敷料材料。我们关注了白蛋白在有过多渗出液伤口处的吸附和释放现象。首先，我们使用分子动力学方法研究了白蛋白动力学，并计算了在实验pH值下水中的静电表面。然后，我们使用不同的pH值和白蛋白浓度，通过吸附和扩散到膜的方法详细研究了果胶-沸石水凝胶。为了了解负载的白蛋白分子在不同配方中是否保持其二级构象，我们使用圆二色-紫外光谱分析监测了pH值和白蛋白浓度对白蛋白从水凝胶中释放后构象变化的影响。我们的结果表明，在pH 6.4时，含牛血清白蛋白的薄膜在蛋白质释放到外部缓冲溶液时保留了蛋白质的折叠结构。体外伤口愈合试验表明，负载白蛋白的水凝胶对成纤维细胞没有毒性作用。
