Department of Polymer Science and Engineering, Kyungpook National University, Daegu, 702-701 South Korea.
Department of Advanced Materials and Chemical Engineering, Catholic University of Daegu, Kyungsan, South Korea.
Biomater Res. 2016 Jul 27;20:23. doi: 10.1186/s40824-016-0071-5. eCollection 2016.
After tumor resection, bone reconstruction such as skull base reconstruction using interconnected porous structure is absolutely necessary. In this study, porous scaffolds for bone reconstruction were prepared using heat-pressing and salt-leaching methods. High-density polyethylene (HDPE) and poly(ethylene-co-acrylic acid) (PEAA) were chosen as the polymer composites for producing a porous scaffold of high mechanical strength and having high reactivity with biomaterials such as collagen, respectively. The porous structure was observed through surface images, and its intrusion volume and porosity were measured. Owing to the carboxylic acids on PEAA, collagen was successfully grafted onto the porous HDPE/PEAA scaffold, which was confirmed by FT-IR spectroscopy and electron spectroscopy for chemical analysis. Osteoblasts were cultured on the collagen-grafted porous scaffold, and their adhesion, proliferation, and differentiation were investigated. The high viability and growth of the osteoblasts suggest that the collagen-grafted porous HDPE/PEAA is a promising scaffold material for bone generation.
肿瘤切除后，使用相互连通的多孔结构进行颅骨底重建等骨重建是绝对必要的。在这项研究中，使用热压和盐浸法制备了用于骨重建的多孔支架。高密度聚乙烯 (HDPE) 和聚 (乙烯-共-丙烯酸) (PEAA) 分别被选为聚合物复合材料，以生产具有高强度机械性能和与胶原蛋白等生物材料高反应性的多孔支架。通过表面图像观察多孔结构，并测量其侵入体积和孔隙率。由于 PEAA 上的羧酸，胶原蛋白成功地接枝到多孔 HDPE/PEAA 支架上，这通过傅里叶变换红外光谱和化学分析电子能谱得到证实。将成骨细胞培养在接枝胶原蛋白的多孔支架上，研究了它们的黏附、增殖和分化。成骨细胞的高存活率和生长表明，接枝胶原蛋白的多孔 HDPE/PEAA 是一种有前途的骨生成支架材料。
