Khomutova Ulyana V, Korzhova Alena G, Bryuzgina Anastasia A, Laput Olesya A, Vasenina Irina V, Akhmadeev Yuriy H, Shugurov Vladimir V, Azhazha Ivan I, Shapovalova Yelena G, Chernyavskii Aleksandr V, Kurzina Irina A
Chemical Department, National Research Tomsk State University, 36 Lenin Ave., Tomsk 634050, Russia.
P.N. Lebedev Physical Institute, 53 Leninsky Prospekt, Moscow 119333, Russia.
Polymers (Basel). 2024 Feb 25;16(5):627. doi: 10.3390/polym16050627.
The effect of surface modification by an arc discharge plasma in a nitrogen flow with treatment durations of 5 and 10 min on the physicochemical properties and biocompatibility of the surface of composites based on polylactic acid and hydroxyapatite (PLA/HA) with different mass ratios (80/20, 70/30, 60/40) has been investigated. The aim of this work was to show the correlation between the changes of the physicochemical characteristics (chemical compound, morphology, wettability) of the surface layer of the PLA/HA composites and the cell viability (macrophages) in the presence of the plasma-modified materials. The dependence of alterations of the functional properties (wettability, biocompatibility) on the change in the chemical composition under the plasma exposure has been established. The chemical composition was studied using X-ray photoelectron spectroscopy (XPS), the surface morphology was researched with scanning electron microscopy (SEM), and the wettability of the composite's surface was analyzed by measuring the contact angle and surface energy calculation. In addition, the viability of macrophages was investigated when the macrophages from three donors interacted with a modified PLA/HA surface. It was found that the formation of the new functional groups, -C-N and N-C=O/C=O, improves the wettability of the surface of the composites and promotes the viability of macrophages in the presence of the composite materials. The fundamental principles for obtaining promising materials with the required properties for eliminating bone defects have been created.
研究了在氮气流中通过电弧放电等离子体进行表面改性，处理时间分别为5分钟和10分钟，对不同质量比（80/20、70/30、60/40）的聚乳酸和羟基磷灰石（PLA/HA）基复合材料表面的物理化学性质和生物相容性的影响。这项工作的目的是展示在存在等离子体改性材料的情况下，PLA/HA复合材料表面层的物理化学特性（化合物、形态、润湿性）变化与细胞活力（巨噬细胞）之间的相关性。已经确定了等离子体暴露下功能特性（润湿性、生物相容性）变化对化学成分变化的依赖性。使用X射线光电子能谱（XPS）研究化学成分，用扫描电子显微镜（SEM）研究表面形态，并通过测量接触角和计算表面能来分析复合材料表面的润湿性。此外，当来自三个供体的巨噬细胞与改性的PLA/HA表面相互作用时，研究了巨噬细胞的活力。发现新官能团-C-N和N-C=O/C=O的形成改善了复合材料表面的润湿性，并在存在复合材料的情况下促进了巨噬细胞的活力。已经确立了获得具有消除骨缺损所需性能的有前景材料的基本原理。
