Department of Dentistry, Taipei Medical University-Shuang Ho Hospital, New Taipei City 235, Taiwan.
Taiwan Society of Blood Biomaterials, New Taipei City 221, Taiwan.
Int J Mol Sci. 2022 Jan 27;23(3):1488. doi: 10.3390/ijms23031488.
The ability of Pluronic F127 (PF127) conjugated with tetrapeptide Gly-Arg-Gly-Asp (GRGD) as a sequence of Arg-Gly-Asp (RGD) peptide to form the investigated potential hydrogel (hereafter referred to as 3DG bioformer (3BE)) to produce spheroid, biocompatibility, and cell invasion ability, was assessed in this study. The fibroblast cell line (NIH 3T3), osteoblast cell line (MG-63), and human breast cancer cell line (MCF-7) were cultured in the 3BE hydrogel and commercial product (Matrigel) for comparison. The morphology of spheroid formation was evaluated via optical microscopy. The cell viability was observed through cell counting Kit-8 assay, and cell invasion was investigated via Boyden chamber assay. Analytical results indicated that 3BE exhibited lower spheroid formation than Matrigel. However, the 3BE appeared biocompatible to NIH 3T3, MG-63, and MCF-7 cells. Moreover, cell invasion ability and cell survival rate after invasion through the 3BE was displayed to be comparable to Matrigel. Thus, these findings demonstrate that the 3BE hydrogel has a great potential as an alternative to a three-dimensional cell culture for drug screening applications.
聚氧乙烯（20）山梨醇酐单月桂酸酯（Pluronic F127，PF127）与四肽甘氨酰-精氨酰-甘氨酰-天冬氨酸（Gly-Arg-Gly-Asp，GRGD）偶联形成所研究的潜在水凝胶（以下简称 3DG 生物形成剂（3BE））的能力，以评估其形成球体、生物相容性和细胞侵袭能力。本研究将成纤维细胞系（NIH 3T3）、成骨细胞系（MG-63）和人乳腺癌细胞系（MCF-7）培养在 3BE 水凝胶和商业产品（Matrigel）中进行比较。通过光学显微镜评估球体形成的形态。通过细胞计数试剂盒-8 测定观察细胞活力，通过 Boyden 室测定研究细胞侵袭。分析结果表明，3BE 形成球体的能力低于 Matrigel。然而，3BE 对 NIH 3T3、MG-63 和 MCF-7 细胞表现出良好的生物相容性。此外，通过 3BE 侵袭后的细胞侵袭能力和细胞存活率显示与 Matrigel 相当。因此，这些发现表明 3BE 水凝胶具有作为药物筛选应用的三维细胞培养替代物的巨大潜力。
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