Tissue Engineering Research Group, Department of Anatomy & Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland.
Cellular and Molecular Imaging Core, Royal College of Surgeons in Ireland (RCSI), 123 St. Stephen's Green, Dublin D02 YN77, Ireland.
ACS Biomater Sci Eng. 2023 Mar 13;9(3):1243-1250. doi: 10.1021/acsbiomaterials.2c01015. Epub 2023 Feb 7.
Materials that are evaluated for bioengineering purposes are carefully tested to evaluate cellular interactions with respect to biocompatibility and in some cases cell differentiation. A key perspective that is often considered is the ability for decellularized synthetic or natural based matrices to facilitate cell migration or tissue ingrowth. Current methods of measuring cell migration range from simple scratch assays to Boyden chamber inserts and fluorescent imaging of seeded spheroids. Many of these methods require tissue processing for histological analysis and fixing and staining for imaging, which can be difficult and dependent on the stability of the hydrogel subject. Herein we present a simple platform that can be manufactured using 3D printing and easily applied to in vitro cell culture, allowing the researcher to image live cellular migration into a cellular materials. We found this to be an adaptable, cheap, and replicable technique to evaluate cellular interaction that has applications in the research and development of hydrogels for tissue engineering purposes.
用于生物工程目的的材料经过精心测试，以评估细胞与生物相容性的相互作用，在某些情况下还评估细胞分化。通常考虑的一个关键观点是脱细胞合成或基于天然的基质促进细胞迁移或组织内生长的能力。目前测量细胞迁移的方法从简单的划痕测定到 Boyden 室插入和接种球体的荧光成像不等。这些方法中的许多方法都需要组织处理进行组织学分析以及固定和染色进行成像，这可能很困难并且取决于水凝胶的稳定性。在此，我们提出了一种简单的平台，可以使用 3D 打印制造，并且可以轻松应用于体外细胞培养，使研究人员能够对活细胞迁移到细胞材料中进行成像。我们发现这是一种适应性强、廉价且可复制的技术，可用于评估细胞相互作用，在组织工程用水凝胶的研究和开发中有应用。
