用于定义染色质中DNA和组蛋白可及性以及长程相互作用的全基因组方法。

Whole-genome methods to define DNA and histone accessibility and long-range interactions in chromatin.

作者信息

Marr Luke T, Jaya Prasoon, Mishra Laxmi N, Hayes Jeffrey J

机构信息

Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY 14642, U.S.A.

Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, U.S.A.

出版信息

Biochem Soc Trans. 2022 Feb 28;50(1):199-212. doi: 10.1042/BST20210959.

DOI:10.1042/BST20210959

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9847230/

Abstract

Defining the genome-wide chromatin landscape has been a goal of experimentalists for decades. Here we review highlights of these efforts, from seminal experiments showing discontinuities in chromatin structure related to gene activation to extensions of these methods elucidating general features of chromatin related to gene states by exploiting deep sequencing methods. We also review chromatin conformational capture methods to identify patterns in long-range interactions between genomic loci.

摘要

几十年来，定义全基因组染色质景观一直是实验人员的目标。在此，我们回顾这些研究工作的要点，从显示与基因激活相关的染色质结构不连续性的开创性实验，到通过利用深度测序方法阐明与基因状态相关的染色质一般特征的这些方法的扩展。我们还回顾了染色质构象捕获方法，以识别基因组位点之间长程相互作用的模式。

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