School of Pharmacy in Minzu University of China, Beijing, 100081, China.
Department of Pharmacy in Zhengzhou Ninth People's Hospital, Zhengzhou, 450000, China.
BMC Med Genomics. 2022 Feb 16;15(1):29. doi: 10.1186/s12920-022-01179-y.
Increased evidence supports the relationship between chromobox protein homolog 3 (CBX3) and tumorigenesis of some cancers. However, the role of CBX3 in pan-cancers remains poorly defined. In the research, we aimed to investigate the prognostic value and the immunological functions of CBX3.
We explored the potential oncogenic roles of CBX3 in mRNA and protein levels based on the diverse databases, including the expression, the correlation with prognosis, tumor microenvironment (TME), DNA methylation, protein phosphorylation and enrichment analysis across all TCGA tumors. The results show that CBX3 is overexpressed in multiple cancers, and significant correlations exist between high expression and adverse prognosis in most tumor patients. We observed an enhanced phosphorylation level in uterine corpus endometrial carcinoma, colon cancer and lung adenocarcinoma. A distinct relationship was also found between CBX3 expression and TME, including immune infiltration of tumor-infiltrating lymphocytes and cancer-associated fibroblasts, immune score or matrix score, immune checkpoints. The correlative transcription factors and miRNAs of CBX3-binding hub genes were analyzed to investigate the molecular mechanism. Moreover, alcoholism and alteration of DNA cellular biology may be involved in the functional mechanisms of CBX3.
The first pan-cancer study offers a relatively comprehensive cognition on the oncogenic roles of CBX3 as a prognostic and immunological marker in various malignant tumors.
越来越多的证据支持 chromobox 蛋白同源物 3（CBX3）与某些癌症的肿瘤发生之间的关系。然而，CBX3 在多种癌症中的作用仍未得到明确界定。在这项研究中，我们旨在研究 CBX3 的预后价值和免疫功能。
我们基于多种数据库，包括表达水平、与预后的相关性、肿瘤微环境（TME）、DNA 甲基化、蛋白磷酸化和全 TCGA 肿瘤的富集分析，探讨了 CBX3 在 mRNA 和蛋白水平上的潜在致癌作用。结果表明，CBX3 在多种癌症中过表达，在大多数肿瘤患者中，高表达与不良预后存在显著相关性。我们观察到在子宫体子宫内膜癌、结肠癌和肺腺癌中磷酸化水平增强。CBX3 表达与 TME 之间也存在明显关系，包括肿瘤浸润淋巴细胞和癌症相关成纤维细胞的免疫浸润、免疫评分或基质评分、免疫检查点。分析了 CBX3 结合枢纽基因的相关转录因子和 miRNA，以研究其分子机制。此外，酒精中毒和 DNA 细胞生物学的改变可能参与了 CBX3 的功能机制。
这是第一项泛癌症研究，提供了关于 CBX3 作为各种恶性肿瘤的预后和免疫标志物的致癌作用的相对全面的认识。
