Fayazipour Dalir, Deckert Joanna, Akbari Gholamali, Soltani Elias, Chmielowska-Bąk Jagna
Department of Agronomy and Plant Breeding Sciences, College of Aboureihan, University of Tehran, Tehran P.O. Box 3391653775, Iran.
Department of Plant Ecophysiology, Institute of Experimental Biology, Faculty of Biology, School of Natural Sciences, Adam Mickiewicz University, 61-712 Poznań, Poland.
Antioxidants (Basel). 2022 Oct 25;11(11):2099. doi: 10.3390/antiox11112099.
Numerous reports find that Cd induces formation of reactive oxygen species (ROS) in plants. However, a general ROS pool is usually studied, without distinction of their production site. In the present study, we applied a mitochondria-specific antioxidant, MitoTEMPO, to elucidate the role of mitochondria-derived ROS in the response of soybean seedlings to short-term (48 h) Cd stress. The obtained results showed that Cd caused a reduction in root length and fresh weight and increase in the level of superoxide anion, hydrogen peroxide, markers of lipid peroxidation (thiobarbituric reactive substances, TBARS) and markers of RNA oxidation (8-hydroxyguanosine, 8-OHG) in seedling roots. Application of MitoTEMPO affected Cd uptake in a dose-dependent manner and diminished the Cd-dependent induction of superoxide anion and lipid peroxidation.
大量报告发现，镉会诱导植物中活性氧（ROS）的形成。然而，通常研究的是总的ROS库，而没有区分它们的产生部位。在本研究中，我们应用了一种线粒体特异性抗氧化剂MitoTEMPO，以阐明线粒体衍生的ROS在大豆幼苗对短期（48小时）镉胁迫反应中的作用。所得结果表明，镉导致幼苗根的根长和鲜重降低，超氧阴离子、过氧化氢、脂质过氧化标志物（硫代巴比妥酸反应性物质，TBARS）和RNA氧化标志物（8-羟基鸟苷，8-OHG）水平升高。MitoTEMPO的应用以剂量依赖的方式影响镉的吸收，并减少了镉依赖性超氧阴离子诱导和脂质过氧化。
