重度早期儿童龋（S-ECC）中咬合面和邻面龋损的特定部位微生物多样性。
Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC).
作者信息
Fakhruddin Kausar Sadia, Samaranayake Lakshman Perera, Hamoudi Rifat Akram, Ngo Hien Chi, Egusa Hiroshi
机构信息
Department of Preventive and Restorative Dentistry, University of Sharjah, Sharjah, UAE.
Division of Molecular and Regenerative Prosthodontics, Tohoku University Graduate School of Dentistry, Sendai-city, Japan.
出版信息
J Oral Microbiol. 2022 Feb 13;14(1):2037832. doi: 10.1080/20002297.2022.2037832. eCollection 2022.
BACKGROUND
Severe-early childhood caries (S-ECC) a global problem of significant concern, commonly manifest on the occlusal, and proximal surfaces of affected teeth. Despite the major ecological differences between these two niches the compositional differences, if any, in the microbiota of such lesions is unknown.
METHODS
Deep-dentine caries samples from asymptomatic primary molars of children with S-ECC (n 19) belonging to caries-code 5/6, (ICDAS classification) were evaluated. Employing two primer pools, we amplified and compared the bacterial gene sequences of the seven hypervariable regions (V2-V4 and V6-V9) using NGS-based assay.
RESULTS
Bray-Curtisevaluation indicated that occlusal lesions (OL) had a more homogeneous community than the proximal lesions (PL) with significant compositional differences at the species level ( = 0.01; R- 0.513). Together, the occlusal and proximal niches harbored 263 species, of which 202 (76.8%) species were common to both , while 49 (18.6%) and 12 (4.6%) disparate species were exclusively isolated from the proximal and occlusal niches, respectively. The most commonl genera at both niches included , and was predominant in PL ( ≤ 0.05), and ( = 0.01) was predominant in OL.
CONCLUSIONS
Distinct differences exist between the caries microbiota of occlusal and proximal caries in S-ECC.
背景
重度幼儿早期龋（S-ECC）是一个全球高度关注的问题，通常表现在患牙的咬合面和邻面。尽管这两个生态位存在重大差异，但此类病变微生物群的组成差异（若有）尚不清楚。
方法
对19名患有S-ECC（龋病编码5/6，国际龋病检测与评估系统分类）的儿童无症状乳磨牙的深龋牙本质样本进行评估。使用两个引物池，我们采用基于二代测序（NGS）的检测方法扩增并比较了七个高变区（V2-V4和V6-V9）的细菌基因序列。
结果
Bray-Curtis评估表明，咬合面病变（OL）的群落比邻面病变（PL）更均匀，在物种水平上存在显著的组成差异（P = 0.01；R = 0.513）。咬合面和邻面生态位共含有263个物种，其中202个（76.8%）物种在两者中都常见，而分别从邻面和咬合面生态位中仅分离出49个（18.6%）和12个（4.6%）不同的物种。两个生态位中最常见的属包括……，且……在PL中占主导（P≤0.05），而……（P = 0.01）在OL中占主导。
结论
S-ECC中咬合面龋和邻面龋的龋病微生物群之间存在明显差异。
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