Bioprocess Engineering, Wageningen University and Research, Wageningen, 6708PB, The Netherlands.
Stichting imec Nederland - OnePlanet Research Center, Wageningen, 6708WH, The Netherlands.
Microb Biotechnol. 2022 Jun;15(6):1824-1838. doi: 10.1111/1751-7915.14013. Epub 2022 Feb 17.
Microalgae industrial production is viewed as a solution for alternative production of nutraceuticals, cosmetics, biofertilizers, and biopolymers. Throughout the years, several technological advances have been implemented, increasing the competitiveness of microalgae industry. However, online monitoring and real-time process control of a microalgae production factory still require further development. In this mini-review, non-destructive tools for online monitoring of cellular agriculture applications are described. Still, the focus is on the use of fluorescence spectroscopy to monitor several parameters (cell concentration, pigments, and lipids) in the microalgae industry. The development presented makes it the most promising solution for monitoring up-and downstream processes, different biological parameters simultaneously, and different microalgae species. The improvements needed for industrial application of this technology are also discussed.
微藻工业生产被视为替代营养保健品、化妆品、生物肥料和生物聚合物生产的一种解决方案。多年来,已经实施了多项技术进步,提高了微藻产业的竞争力。然而,微藻生产工厂的在线监测和实时过程控制仍需要进一步发展。在这篇迷你综述中,描述了用于细胞农业应用在线监测的非破坏性工具。不过,重点是使用荧光光谱法来监测微藻工业中的几个参数(细胞浓度、色素和脂质)。所提出的发展使其成为监测上下游过程、同时监测不同生物参数和不同微藻物种的最有前途的解决方案。还讨论了将这项技术应用于工业生产所需的改进。